Gene description for CYTH3
Gene name cytohesin 3
Gene symbol CYTH3
Other names/aliases ARNO3
GRP1
PSCD3
Species Homo sapiens
 Database cross references - CYTH3
ExoCarta ExoCarta_9265
Entrez Gene 9265
HGNC 9504
MIM 605081
UniProt O43739  
 CYTH3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CYTH3
Molecular Function
    ARF guanyl-nucleotide exchange factor activity GO:0005086 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
Biological Process
    establishment of epithelial cell polarity GO:0090162 IEA
    positive regulation of cell adhesion GO:0045785 IEA
    Golgi vesicle transport GO:0048193 IMP
    regulation of cell adhesion GO:0030155 IBA
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    trans-Golgi network GO:0005802 IBA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IDA
    plasma membrane GO:0005886 IDA
    ruffle GO:0001726 IEA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified CYTH3 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CYTH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALCOCO1  
Two-hybrid Homo sapiens
2 IPCEF1 26034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 CNKSR1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CYTH3 is involved
No pathways found





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