Gene description for Eftud2
Gene name elongation factor Tu GTP binding domain containing 2
Gene symbol Eftud2
Other names/aliases 116kDa
Snrp116
U5-116kD
Species Mus musculus
 Database cross references - Eftud2
ExoCarta ExoCarta_20624
Vesiclepedia VP_20624
Entrez Gene 20624
UniProt O08810  
 Eftud2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eftud2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    U5 snRNA binding GO:0030623 IBA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    Cajal body GO:0015030 ISO
    nuclear speck GO:0016607 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    U2-type catalytic step 2 spliceosome GO:0071007 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISS
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Eftud2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eftud2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
4 Ruvbl2 20174
Co-fractionation Mus musculus
5 Sars 20226
Co-fractionation Mus musculus
6 Snrpd1 20641
Co-fractionation Mus musculus
7 Srrm1 51796
Co-fractionation Mus musculus
8 Prpf19 28000
Co-fractionation Mus musculus
9 Tubb5 22154
Co-fractionation Mus musculus
10 Sart3  
Co-fractionation Mus musculus
11 Wdr5  
Co-fractionation Mus musculus
12 Alyref  
Co-fractionation Mus musculus
13 Rbbp4 19646
Co-fractionation Mus musculus
14 Snrpd2 107686
Co-fractionation Mus musculus
15 Atxn1  
Affinity Capture-MS Mus musculus
16 Snrnp200 320632
Co-fractionation Mus musculus
17 Frg1  
Co-fractionation Mus musculus
18 Rbbp7 245688
Co-fractionation Mus musculus
19 Chaf1a  
Affinity Capture-MS Mus musculus
20 Ttc1  
Co-fractionation Mus musculus
21 Snrpd3 67332
Co-fractionation Mus musculus
22 Sf3b3 101943
Co-fractionation Mus musculus
23 Rbm8a  
Co-fractionation Mus musculus
24 Lsm3  
Co-fractionation Mus musculus
25 Pde4dip  
Affinity Capture-MS Mus musculus
26 Srsf1 110809
Co-fractionation Mus musculus
27 Plrg1  
Co-fractionation Mus musculus
28 Hnrnpc 15381
Co-fractionation Mus musculus
29 Sh3glb2  
Co-fractionation Mus musculus
30 Rpap3  
Co-fractionation Mus musculus
31 Srrm2  
Co-fractionation Mus musculus
32 Syncrip 56403
Co-fractionation Mus musculus
33 Snw1  
Co-fractionation Mus musculus
34 Ppwd1  
Co-fractionation Mus musculus
35 Sfpq 71514
Co-fractionation Mus musculus
36 Zbtb7b  
Affinity Capture-MS Mus musculus
37 Sf3b1 81898
Co-fractionation Mus musculus
38 Ppil3  
Co-fractionation Mus musculus
39 Eed  
Affinity Capture-MS Mus musculus
40 Cwc27  
Co-fractionation Mus musculus
41 Prpf4  
Co-fractionation Mus musculus
42 Skiv2l2  
Co-fractionation Mus musculus
43 Eif4a3 192170
Co-fractionation Mus musculus
44 Prpf31  
Co-fractionation Mus musculus
45 Fancd2  
Affinity Capture-MS Mus musculus
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