Gene description for Banf1
Gene name barrier to autointegration factor 1
Gene symbol Banf1
Other names/aliases Baf
Bcrp1
L2bp1/Baf
Species Mus musculus
 Database cross references - Banf1
ExoCarta ExoCarta_23825
Vesiclepedia VP_23825
Entrez Gene 23825
UniProt O54962  
 Banf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Banf1
Molecular Function
    DNA binding GO:0003677 IBA
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 TAS
    double-stranded DNA binding GO:0003690 IEA
    double-stranded DNA binding GO:0003690 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    chromatin organization GO:0006325 ISO
    chromatin organization GO:0006325 ISS
    response to oxidative stress GO:0006979 ISO
    response to oxidative stress GO:0006979 ISS
    mitotic nuclear membrane reassembly GO:0007084 ISO
    mitotic nuclear membrane reassembly GO:0007084 ISS
    negative regulation of protein ADP-ribosylation GO:0010836 ISO
    negative regulation of protein ADP-ribosylation GO:0010836 ISS
    DNA integration GO:0015074 IDA
    negative regulation of type I interferon production GO:0032480 ISO
    negative regulation of type I interferon production GO:0032480 ISS
    negative regulation of viral genome replication GO:0045071 IEA
    negative regulation of viral genome replication GO:0045071 ISO
    negative regulation of innate immune response GO:0045824 IEA
    negative regulation of innate immune response GO:0045824 ISO
    chromosome organization GO:0051276 IBA
    establishment of integrated proviral latency GO:0075713 TAS
    negative regulation of cGAS/STING signaling pathway GO:0160049 IEA
    negative regulation of cGAS/STING signaling pathway GO:0160049 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    condensed chromosome GO:0000793 IBA
    condensed chromosome GO:0000793 IEA
    condensed chromosome GO:0000793 ISO
    condensed chromosome GO:0000793 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 IEA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Banf1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Banf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Foxl2  
Two-hybrid Mus musculus
3 Tufm  
Co-fractionation Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Atg16l1  
Affinity Capture-MS Mus musculus
6 Snrpd3 67332
Co-fractionation Mus musculus
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