Gene description for SIGIRR
Gene name single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
Gene symbol SIGIRR
Other names/aliases TIR8
Species Homo sapiens
 Database cross references - SIGIRR
ExoCarta ExoCarta_59307
Vesiclepedia VP_59307
Entrez Gene 59307
HGNC 30575
MIM 605478
UniProt Q6IA17  
 SIGIRR identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for SIGIRR
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of cytokine-mediated signaling pathway GO:0001960 ISS
    acute-phase response GO:0006953 ISS
    cell surface receptor signaling pathway GO:0007166 IBA
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 TAS
    negative regulation of chemokine production GO:0032682 ISS
    negative regulation of Toll signaling pathway GO:0045751 IMP
    negative regulation of interleukin-1-mediated signaling pathway GO:2000660 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    membrane GO:0016020 IMP
 Experiment description of studies that identified SIGIRR in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SIGIRR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 ENO2 2026
Two-hybrid Homo sapiens
3 IRAK1  
Affinity Capture-Western Homo sapiens
4 LGALS9 3965
Affinity Capture-MS Homo sapiens
5 MYCN  
Affinity Capture-MS Homo sapiens
6 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 LGALS8 3964
Affinity Capture-MS Homo sapiens
8 TLR4  
Affinity Capture-Western Homo sapiens
9 SIGIRR 59307
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 IL1R1 3554
Affinity Capture-Western Homo sapiens
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