Gene description for RRAGD
Gene name Ras-related GTP binding D
Gene symbol RRAGD
Other names/aliases RAGD
bA11D8.2.1
Species Homo sapiens
 Database cross references - RRAGD
ExoCarta ExoCarta_58528
Vesiclepedia VP_58528
Entrez Gene 58528
HGNC 19903
MIM 608268
UniProt Q9NQL2  
 RRAGD identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for RRAGD
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IMP
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 IMP
    protein heterodimerization activity GO:0046982 IPI
    GTPase binding GO:0051020 IPI
    molecular adaptor activity GO:0060090 IDA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    protein localization GO:0008104 ISS
    cellular response to starvation GO:0009267 IBA
    negative regulation of autophagy GO:0010507 IBA
    positive regulation of TOR signaling GO:0032008 NAS
    cellular response to amino acid stimulus GO:0071230 ISS
    cellular response to L-leucine GO:0071233 IMP
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IMP
    cellular response to leucine starvation GO:1990253 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    Gtr1-Gtr2 GTPase complex GO:1990131 IBA
 Experiment description of studies that identified RRAGD in exosomes
1
Experiment ID 255
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RRAGD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LARS 51520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 KDF1  
Affinity Capture-MS Homo sapiens
3 FLCN 201163
Affinity Capture-Western Homo sapiens
4 GNGT1  
Affinity Capture-MS Homo sapiens
5 NOL8  
Affinity Capture-Western Homo sapiens
6 LAMTOR4 389541
Affinity Capture-MS Homo sapiens
7 KRAS 3845
Proximity Label-MS Homo sapiens
8 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
10 NPRL2  
Affinity Capture-Western Homo sapiens
11 LAMTOR2 28956
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 TRIM16 10626
Affinity Capture-Western Homo sapiens
14 LAMTOR3 8649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 RRAGC 64121
Affinity Capture-Western Homo sapiens
Negative Genetic Homo sapiens
Affinity Capture-Western Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 RRAGA 10670
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TSC2 7249
Affinity Capture-Western Homo sapiens
19 RAB9A 9367
Proximity Label-MS Homo sapiens
20 MTOR 2475
Affinity Capture-Western Homo sapiens
21 RRAGB 10325
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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