Gene description for PLCB4
Gene name phospholipase C, beta 4
Gene symbol PLCB4
Other names/aliases ARCND2
PI-PLC
Species Homo sapiens
 Database cross references - PLCB4
ExoCarta ExoCarta_5332
Entrez Gene 5332
HGNC 9059
MIM 600810
UniProt Q15147  
 PLCB4 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for PLCB4
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IEA
    phospholipase C activity GO:0004629 TAS
    signal transducer activity GO:0004871 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    inositol phosphate metabolic process GO:0043647 TAS
    intracellular signal transduction GO:0035556 IEA
    small molecule metabolic process GO:0044281 TAS
    lipid catabolic process GO:0016042 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    postsynaptic density GO:0014069 IEA
    smooth endoplasmic reticulum GO:0005790 IEA
    nucleus GO:0005634 IEA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified PLCB4 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for PLCB4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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