Gene description for PAM
Gene name peptidylglycine alpha-amidating monooxygenase
Gene symbol PAM
Other names/aliases PAL
PHM
Species Homo sapiens
 Database cross references - PAM
ExoCarta ExoCarta_5066
Entrez Gene 5066
HGNC 8596
MIM 170270
UniProt P19021  
 PAM identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25857301    
Saliva 19199708    
 Gene ontology annotations for PAM
Molecular Function
    peptidylglycine monooxygenase activity GO:0004504 IDA
    L-ascorbic acid binding GO:0031418 IEA
    copper ion binding GO:0005507 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    peptidylamidoglycolate lyase activity GO:0004598 IDA
Biological Process
    oxidation-reduction process GO:0055114 IEA
    peptide amidation GO:0001519 IDA
Subcellular Localization
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified PAM in exosomes
1
Experiment ID 250
ISEV standards
EM
EV Biophysical techniques
GAPDH|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PAMCI  
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 TSC2 7249
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
3 KALRN 8997
Two-hybrid Homo sapiens
4 TSC1  
Co-localization Homo sapiens
View the network image/svg+xml
 Pathways in which PAM is involved
No pathways found





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