Gene description for IRF2
Gene name interferon regulatory factor 2
Gene symbol IRF2
Other names/aliases IRF-2
Species Homo sapiens
 Database cross references - IRF2
ExoCarta ExoCarta_3660
Entrez Gene 3660
HGNC 6117
MIM 147576
UniProt P14316  
 IRF2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for IRF2
Molecular Function
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IMP
    DNA binding GO:0003677 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IMP
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001228 IMP
    protein binding GO:0005515 IPI
Biological Process
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    type I interferon signaling pathway GO:0060337 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    blood coagulation GO:0007596 TAS
    cell proliferation GO:0008283 TAS
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    regulation of transcription, DNA-templated GO:0006355 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    focal adhesion GO:0005925 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IRF2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for IRF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NFKB1 4790
Invitro Homo sapiens
2 GCN5L2  
Reconstituted Complex Homo sapiens
3 BRD7  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 PRKACA 5566
Biochemical Activity Homo sapiens
5 RELA  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 PCAF  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CSNK2A1 1457
Biochemical Activity Homo sapiens
8 IRF8  
Invivo Homo sapiens
Invitro Homo sapiens
9 EP300 2033
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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