Gene description for LAMA1
Gene name laminin, alpha 1
Gene symbol LAMA1
Other names/aliases LAMA
PTBHS
S-LAM-alpha
Species Homo sapiens
 Database cross references - LAMA1
ExoCarta ExoCarta_284217
Entrez Gene 284217
HGNC 6481
MIM 150320
UniProt P25391  
 LAMA1 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for LAMA1
Molecular Function
    extracellular matrix structural constituent GO:0005201 ISS
    receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    glycosphingolipid binding GO:0043208 IEA
Biological Process
    cell adhesion GO:0007155 IEA
    extracellular matrix organization GO:0030198 TAS
    regulation of cell adhesion GO:0030155 IEA
    retinal blood vessel morphogenesis GO:0061304 IEA
    morphogenesis of an epithelial sheet GO:0002011 IEA
    regulation of cell migration GO:0030334 IEA
    axon guidance GO:0007411 TAS
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    regulation of embryonic development GO:0045995 IEA
    cell surface receptor signaling pathway GO:0007166 ISS
    branching involved in salivary gland morphogenesis GO:0060445 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 IEA
Subcellular Localization
    cell-cell junction GO:0005911 IEA
    extracellular region GO:0005576 TAS
    laminin-3 complex GO:0005608 IPI
    extracellular space GO:0005615 IDA
    proteinaceous extracellular matrix GO:0005578 ISS
    laminin-1 complex GO:0005606 IPI
    extracellular matrix GO:0031012 TAS
    basement membrane GO:0005604 IDA
 Experiment description of studies that identified LAMA1 in exosomes
1
Experiment ID 254
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LAMA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Invitro Homo sapiens
2 MEP1A 4224
Biochemical Activity Homo sapiens
3 HSPG2 3339
Invitro Homo sapiens
4 ITGA2 3673
Invitro Homo sapiens
5 PLAT 5327
Invitro Homo sapiens
6 PLG 5340
Invitro Homo sapiens
7 FBLN2 2199
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
8 ITGA1 3672
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which LAMA1 is involved
PathwayEvidenceSource
ECM proteoglycans TAS Reactome
L1CAM interactions TAS Reactome
Laminin interactions TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome





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