Gene description for LAMA1
Gene name laminin, alpha 1
Gene symbol LAMA1
Other names/aliases LAMA
PTBHS
S-LAM-alpha
Species Homo sapiens
 Database cross references - LAMA1
ExoCarta ExoCarta_284217
Vesiclepedia VP_284217
Entrez Gene 284217
HGNC 6481
MIM 150320
UniProt P25391  
 LAMA1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LAMA1
Molecular Function
    signaling receptor binding GO:0005102 IEA
    extracellular matrix structural constituent GO:0005201 ISS
    protein binding GO:0005515 IPI
    glycosphingolipid binding GO:0043208 IEA
Biological Process
    morphogenesis of an epithelial sheet GO:0002011 IEA
    cell adhesion GO:0007155 IEA
    cell surface receptor signaling pathway GO:0007166 ISS
    regulation of cell migration GO:0030334 IEA
    neuron projection development GO:0031175 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 IEA
    positive regulation of cell adhesion GO:0045785 NAS
    regulation of embryonic development GO:0045995 IEA
    positive regulation of muscle cell differentiation GO:0051149 NAS
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    branching involved in salivary gland morphogenesis GO:0060445 IEA
    retinal blood vessel morphogenesis GO:0061304 IEA
    regulation of basement membrane organization GO:0110011 NAS
    positive regulation of integrin-mediated signaling pathway GO:2001046 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 NAS
    laminin-1 complex GO:0005606 IDA
    laminin-1 complex GO:0005606 IPI
    laminin-3 complex GO:0005608 IPI
    extracellular space GO:0005615 IDA
    cell-cell junction GO:0005911 IEA
    membrane GO:0016020 IDA
    extracellular matrix GO:0031012 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 TAS
    protein complex involved in cell-matrix adhesion GO:0098637 NAS
 Experiment description of studies that identified LAMA1 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LAMA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
2 CBX4  
Affinity Capture-MS Homo sapiens
3 TOMM40 10452
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-MS Homo sapiens
5 MEP1A 4224
Biochemical Activity Homo sapiens
6 NID2 22795
Affinity Capture-MS Homo sapiens
7 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Myh3  
Affinity Capture-MS Mus musculus
9 HSPA5 3309
Affinity Capture-MS Homo sapiens
10 Prkci  
Affinity Capture-MS Mus musculus
11 PLAT 5327
Reconstituted Complex Homo sapiens
12 Trim32  
Affinity Capture-MS Mus musculus
13 NES 10763
Affinity Capture-MS Homo sapiens
14 HSPG2 3339
Reconstituted Complex Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 TUBG1 7283
Affinity Capture-MS Homo sapiens
17 PLG 5340
Reconstituted Complex Homo sapiens
18 SUCO  
Affinity Capture-MS Homo sapiens
19 CCDC132 55610
Affinity Capture-MS Homo sapiens
20 KRT35 3886
Affinity Capture-MS Homo sapiens
21 ATL2 64225
Affinity Capture-MS Homo sapiens
22 ITGB1 3688
Reconstituted Complex Homo sapiens
23 ITGA1 3672
Reconstituted Complex Homo sapiens
24 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PDGFRA 5156
Affinity Capture-MS Homo sapiens
26 MANEA 79694
Affinity Capture-MS Homo sapiens
27 STRN3 29966
Affinity Capture-MS Homo sapiens
28 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
29 ARRDC1 92714
Affinity Capture-MS Homo sapiens
30 FBXO2 26232
Affinity Capture-MS Homo sapiens
31 OS9 10956
Affinity Capture-MS Homo sapiens
32 LGALS7 3963
Affinity Capture-MS Homo sapiens
33 CCL3  
Affinity Capture-MS Homo sapiens
34 ITGA2 3673
Reconstituted Complex Homo sapiens
35 Immt  
Affinity Capture-MS Mus musculus
36 ZNF408  
Affinity Capture-MS Homo sapiens
37 Tubg1 103733
Affinity Capture-MS Mus musculus
38 Get4  
Affinity Capture-MS Mus musculus
39 LCN9  
Affinity Capture-MS Homo sapiens
40 MAPK9 5601
Affinity Capture-MS Homo sapiens
41 SCGB2A2  
Affinity Capture-MS Homo sapiens
42 C1orf54  
Affinity Capture-MS Homo sapiens
43 Tube1  
Affinity Capture-MS Mus musculus
44 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 LAMB3 3914
Affinity Capture-MS Homo sapiens
46 Bag2  
Affinity Capture-MS Mus musculus
47 Prmt6  
Affinity Capture-MS Mus musculus
48 Kctd5  
Affinity Capture-MS Mus musculus
49 FBLN2 2199
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
50 AP4E1  
Affinity Capture-MS Homo sapiens
51 FGF4  
Affinity Capture-MS Homo sapiens
52 C1QTNF7  
Affinity Capture-MS Homo sapiens
53 Dctn4  
Affinity Capture-MS Mus musculus
54 IGFL3  
Affinity Capture-MS Homo sapiens
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