Gene description for ITGA1
Gene name integrin, alpha 1
Gene symbol ITGA1
Other names/aliases CD49a
VLA1
Species Homo sapiens
 Database cross references - ITGA1
ExoCarta ExoCarta_3672
Entrez Gene 3672
HGNC 6134
MIM 192968
UniProt P56199  
 ITGA1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for ITGA1
Molecular Function
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 IEA
    collagen binding involved in cell-matrix adhesion GO:0098639 IMP
    collagen binding GO:0005518 TAS
    protein phosphatase binding GO:0019903 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    neutrophil chemotaxis GO:0030593 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    cellular extravasation GO:0045123 IEA
    activation of MAPK activity GO:0000187 IEA
    integrin-mediated signaling pathway GO:0007229 IEA
    positive regulation of phosphoprotein phosphatase activity GO:0032516 IDA
    cell-matrix adhesion GO:0007160 IMP
    axon guidance GO:0007411 TAS
    blood coagulation GO:0007596 TAS
    muscle contraction GO:0006936 TAS
    vasodilation GO:0042311 IEA
    negative regulation of cell proliferation GO:0008285 IMP
    extracellular matrix organization GO:0030198 TAS
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 ISS
Subcellular Localization
    focal adhesion GO:0005925 IDA
    perikaryon GO:0043204 IEA
    membrane raft GO:0045121 IEA
    extracellular exosome GO:0070062 IDA
    external side of plasma membrane GO:0009897 IEA
    integrin alpha1-beta1 complex GO:0034665 IDA
    neuron projection GO:0043005 IEA
    basal part of cell GO:0045178 IEA
    cell surface GO:0009986 IDA
    acrosomal vesicle GO:0001669 IEA
    integrin complex GO:0008305 TAS
    membrane GO:0016020 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified ITGA1 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ITGA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MATN1  
Invivo Homo sapiens
Invitro Homo sapiens
2 COL13A1  
Invivo Homo sapiens
3 AUP1  
Two-hybrid Homo sapiens
4 LAMA1 284217
Invitro Homo sapiens
5 BIN1 274
Two-hybrid Homo sapiens
6 TLN1 7094
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here