Gene description for Tsg101
Gene name tumor susceptibility gene 101
Gene symbol Tsg101
Other names/aliases AI255943
CC2
Species Mus musculus
 Database cross references - Tsg101
ExoCarta ExoCarta_22088
Vesiclepedia VP_22088
Entrez Gene 22088
UniProt Q61187  
 Tsg101 identified in sEVs derived from the following tissue/cell type
Adipocytes 36640325    
Adipocytes 36640325    
Breast cancer cells 34112803    
Breast cancer cells 34112803    
Colon cancer cells 37309723    
Dendritic cells 11390481    
Endothelial cells 34887361    
Endothelial cells 34887361    
Hepatocytes 19367702    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hepatocytes 34489604    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Hippocampal neuronal cells 30967557    
Kidney medullary cells 34580290    
Lung cancer cells 36270983    
Lung epithelial cells 36270983    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Melanoma cells 30089911    
Mesenchymal stem cells 36260670    
Mesenchymal stem cells 36253358    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic cancer cells 33827783    
Pancreatic duct epithelial cells 33827783    
Pancreatic duct epithelial cells 33827783    
Pancreatic duct epithelial cells 33827783    
Podocytes 30967557    
Podocytes 30967557    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tsg101
Molecular Function
    transcription corepressor activity GO:0003714 IDA
    protein binding GO:0005515 IPI
    nuclear receptor coactivator activity GO:0030374 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    virion binding GO:0046790 IEA
    virion binding GO:0046790 ISO
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of cell growth GO:0001558 IDA
    protein monoubiquitination GO:0006513 ISO
    extracellular transport GO:0006858 IEA
    extracellular transport GO:0006858 ISO
    negative regulation of epidermal growth factor-activated receptor activity GO:0007175 ISO
    negative regulation of cell population proliferation GO:0008285 IDA
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 ISO
    protein transport GO:0015031 IEA
    viral release from host cell GO:0019076 IEA
    viral release from host cell GO:0019076 ISO
    cell differentiation GO:0030154 IDA
    keratinocyte differentiation GO:0030216 IDA
    protein modification process GO:0036211 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISS
    regulation of MAP kinase activity GO:0043405 ISO
    viral budding GO:0046755 IMP
    viral budding GO:0046755 ISO
    cell division GO:0051301 IEA
    regulation of cell cycle GO:0051726 IDA
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    regulation of extracellular exosome assembly GO:1903551 IEA
    regulation of extracellular exosome assembly GO:1903551 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 IEA
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    exosomal secretion GO:1990182 IMP
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 IMP
Subcellular Localization
    ESCRT I complex GO:0000813 IBA
    ESCRT I complex GO:0000813 ISO
    ESCRT I complex GO:0000813 ISS
    ESCRT I complex GO:0000813 NAS
    nucleus GO:0005634 IC
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endosome membrane GO:0010008 ISO
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IEA
    extracellular exosome GO:0070062 IDA
    extracellular exosome GO:0070062 ISO
    Flemming body GO:0090543 IEA
 Experiment description of studies that identified Tsg101 in sEVs
1
Experiment ID 655
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36640325    
Organism Mus musculus
Experiment description Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis
Authors "Tang Y, Yang LJ, Liu H, Song YJ, Yang QQ, Liu Y, Qian SW, Tang QQ."
Journal name Cell Rep
Publication year 2023
Sample Adipocytes
Sample name Visceral (gonadal) adipose tissue
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
2
Experiment ID 656
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36640325    
Organism Mus musculus
Experiment description Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis
Authors "Tang Y, Yang LJ, Liu H, Song YJ, Yang QQ, Liu Y, Qian SW, Tang QQ."
Journal name Cell Rep
Publication year 2023
Sample Adipocytes
Sample name Visceral (gonadal) adipose tissue
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
3
Experiment ID 520
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name EO771
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 521
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd9
Enriched markers
✔
Golga2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34112803    
Organism Mus musculus
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Breast cancer cells
Sample name PyMT
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 10
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|CD9|MHCII|MHCI|CD86|LAMP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QTOF]
PubMed ID 11390481    
Organism Mus musculus
Experiment description Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors "Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S."
Journal name JIMMU
Publication year 2001
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
8
Experiment ID 1089
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887361    
Organism Mus musculus
Experiment description Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Authors "Wang S, Shi M, Li J, Zhang Y, Wang W, Xu P, Li Y."
Journal name Aging
Publication year 2021
Sample Endothelial cells
Sample name Mouse aortic endothelial cells (MAEC)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 1090
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887361    
Organism Mus musculus
Experiment description Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Authors "Wang S, Shi M, Li J, Zhang Y, Wang W, Xu P, Li Y."
Journal name Aging
Publication year 2021
Sample Endothelial cells
Sample name Mouse aortic endothelial cells (MAEC)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 36
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Mus musculus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name MLP-29
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 695
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
12
Experiment ID 696
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
13
Experiment ID 697
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Sdcbp
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
14
Experiment ID 698
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - hepatocyte-specific Cas9 transgenic C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
qRT-PCR
15
Experiment ID 699
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34489604    
Organism Mus musculus
Experiment description Hepatocyte-derived exosomes from early onset obese mice promote insulin sensitivity through miR-3075
Authors "Ji Y, Luo Z, Gao H, Dos Reis FCG, Bandyopadhyay G, Jin Z, Manda KA, Isaac R, Yang M, Fu W, Ying W, Olefsky JM. "
Journal name Nat Metab
Publication year 2021
Sample Hepatocytes
Sample name Primary hepatocytes - hepatocyte-specific Cas9 transgenic C57BL/6
Isolation/purification methods Centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
qRT-PCR
16
Experiment ID 1048
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 1049
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 1050
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 1051
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 1065
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 1066
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Hippocampal neuronal cells
Sample name HT22
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 1149
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34580290    
Organism Mus musculus
Experiment description Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Authors "Volz AK, Frei A, Kretschmer V, de Jesus Domingues AM, Ketting RF, Ueffing M, Boldt K, Krämer-Albers EM, May-Simera HL."
Journal name Nat Commun
Publication year 2021
Sample Kidney medullary cells
Sample name Kidney medullary cells - ciliated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
small RNA sequencing
23
Experiment ID 502
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36270983    
Organism Mus musculus
Experiment description NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer
Authors "Rao X, Zhou X, Wang G, Jie X, Xing B, Xu Y, Chen Y, Li J, Zhu K, Wu Z, Wu G, Wu C, Zhou R."
Journal name Cell Death Dis
Publication year 2022
Sample Lung cancer cells
Sample name Small cell lung cancer (SCLC)
Isolation/purification methods Centrifugation
Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 503
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Cd81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36270983    
Organism Mus musculus
Experiment description NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer
Authors "Rao X, Zhou X, Wang G, Jie X, Xing B, Xu Y, Chen Y, Li J, Zhu K, Wu Z, Wu G, Wu C, Zhou R."
Journal name Cell Death Dis
Publication year 2022
Sample Lung epithelial cells
Sample name Normal lung epithelium
Isolation/purification methods Centrifugation
Exosome Isolation Reagent
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 968
MISEV standards
✘ Biophysical techniques
✔
Tsg101|Gapdh
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30089911    
Organism Mus musculus
Experiment description Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Authors "Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W."
Journal name Nature
Publication year 2018
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 499
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36260670    
Organism Mus musculus
Experiment description "Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old mice"
Authors "Sanz-Ros J, Romero-García N, Mas-Bargues C, Monleón D, Gordevicius J, Brooke RT, Dromant M, Díaz A, Derevyanko A, Guío-Carrión A, Román-Domínguez A, Inglés M, Blasco MA, Horvath S, Viña J, Borrás C. "
Journal name Sci Adv
Publication year 2022
Sample Mesenchymal stem cells
Sample name Adipose MSCs from young C57BL/6J mice
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Flow cytometry
Immuno-EM
miRNA array
30
Experiment ID 504
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Cd63|Cd9
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36253358    
Organism Mus musculus
Experiment description Bone marrow mesenchymal stem cell-derived exosomal miR-21a-5p alleviates renal fibrosis by attenuating glycolysis by targeting PFKM
Authors "Xu S, Cheuk YC, Jia Y, Chen T, Chen J, Luo Y, Cao Y, Guo J, Dong L, Zhang Y, Shi Y, Rong R."
Journal name Cell Death Dis
Publication year 2022
Sample Mesenchymal stem cells
Sample name Bone marrow-derived MSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
miRNA sequencing
qPCR
31
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
32
Experiment ID 262
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|HSP90|CD63|CD9|
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
33
Experiment ID 1020
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
34
Experiment ID 1021
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
35
Experiment ID 1022
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1023
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
37
Experiment ID 1034
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
38
Experiment ID 1035
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
39
Experiment ID 1036
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
40
Experiment ID 1037
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
41
Experiment ID 1038
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
42
Experiment ID 1039
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
43
Experiment ID 1040
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
44
Experiment ID 1041
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
45
Experiment ID 1042
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
46
Experiment ID 1043
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name Panc02
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
47
Experiment ID 1044
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
48
Experiment ID 1045
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Homo sapiens
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic cancer cells
Sample name KPC
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
49
Experiment ID 1019
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
50
Experiment ID 1046
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
51
Experiment ID 1047
MISEV standards
✘ Biophysical techniques
✔
Tsg101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33827783    
Organism Mus musculus
Experiment description Exosome-delivered CD44v6/C1QBP complex drives pancreatic cancer liver metastasis by promoting fibrotic liver microenvironment
Authors "Xie Z, Gao Y, Ho C, Li L, Jin C, Wang X, Zou C, Mao Y, Wang X, Li Q, Fu D, Zhang YF."
Journal name Gut
Publication year 2022
Sample Pancreatic duct epithelial cells
Sample name Primary pancreatic duct epithelial cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
52
Experiment ID 1052
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
53
Experiment ID 1053
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101|Cd9
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30967557    
Organism Mus musculus
Experiment description KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors "Song L, Tang S, Han X, Jiang Z, Dong L, Liu C, Liang X, Dong J, Qiu C, Wang Y, Du Y."
Journal name Nat Commun
Publication year 2019
Sample Podocytes
Sample name MPC-5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
54
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
55
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tsg101
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 CHMP3 51652
Affinity Capture-MS Homo sapiens
3 Vps28 66914
Co-fractionation Mus musculus
4 Gja1 14609
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Atxn2 20239
Co-fractionation Mus musculus
6 Nr3c2  
Affinity Capture-Western Mus musculus
7 Gjc1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 VPS28 51160
Affinity Capture-MS Homo sapiens
9 Gjb3  
Affinity Capture-Western Mus musculus
10 ERLIN1 10613
Affinity Capture-MS Homo sapiens
11 Ndfip1  
Co-fractionation Mus musculus
12 VPS37C 55048
Affinity Capture-MS Homo sapiens
13 Ikbkg  
Two-hybrid Mus musculus
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Pigq  
Co-fractionation Mus musculus
16 MVB12A 93343
Affinity Capture-MS Homo sapiens
17 Dzip3  
Co-fractionation Mus musculus
18 Mgrn1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
19 CYBRD1 79901
Affinity Capture-MS Homo sapiens
20 Tom1 21968
Co-fractionation Mus musculus
21 VPS37A 137492
Affinity Capture-MS Homo sapiens
22 IST1 9798
Affinity Capture-MS Homo sapiens
23 CHMP4B 128866
Affinity Capture-MS Homo sapiens
24 Birc2  
Co-fractionation Mus musculus
25 Cope  
Co-fractionation Mus musculus
26 CHMP5 51510
Affinity Capture-MS Homo sapiens
27 Gmcl1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
28 UBAP1 51271
Affinity Capture-MS Homo sapiens
29 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
30 Abcb6  
Co-fractionation Mus musculus
31 Gjd3  
Affinity Capture-Western Mus musculus
32 CHMP2B 25978
Affinity Capture-MS Homo sapiens
33 FLOT2 2319
Affinity Capture-MS Homo sapiens
34 CAV1 857
Affinity Capture-MS Homo sapiens
35 Tsg101 22088
Two-hybrid Mus musculus
Two-hybrid Mus musculus
36 Nfe2  
Two-hybrid Mus musculus
37 Ppp1cc 19047
Affinity Capture-Western Mus musculus
38 VPS37B 79720
Affinity Capture-MS Homo sapiens
39 GPRC5A 9052
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here