Gene description for Ppp1cc
Gene name protein phosphatase 1, catalytic subunit, gamma isoform
Gene symbol Ppp1cc
Other names/aliases PP-1G
PP1
dis2m1
Species Mus musculus
 Database cross references - Ppp1cc
ExoCarta ExoCarta_19047
Vesiclepedia VP_19047
Entrez Gene 19047
UniProt P63087  
 Ppp1cc identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp1cc
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein serine/threonine phosphatase activity GO:0004722 IMP
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 IEA
    lamin binding GO:0005521 ISO
    protein phosphatase 1 binding GO:0008157 IEA
    protein phosphatase 1 binding GO:0008157 ISO
    phosphatase activity GO:0016791 IDA
    myosin phosphatase activity GO:0017018 IEA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    blastocyst development GO:0001824 IMP
    glycogen metabolic process GO:0005977 IEA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 ISO
    spermatogenesis GO:0007283 IMP
    neuron differentiation GO:0030182 ISS
    circadian regulation of gene expression GO:0032922 IDA
    regulation of circadian rhythm GO:0042752 IDA
    regulation of circadian rhythm GO:0042752 IMP
    regulation of circadian rhythm GO:0042752 ISO
    entrainment of circadian clock by photoperiod GO:0043153 IMP
    regulation of nucleocytoplasmic transport GO:0046822 IMP
    cell division GO:0051301 IEA
    positive regulation of glial cell proliferation GO:0060252 IEA
    positive regulation of glial cell proliferation GO:0060252 ISO
Subcellular Localization
    kinetochore GO:0000776 IEA
    chromosome, telomeric region GO:0000781 IEA
    chromosome, telomeric region GO:0000781 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IDA
    microtubule organizing center GO:0005815 IEA
    nuclear speck GO:0016607 IEA
    midbody GO:0030496 IEA
    cleavage furrow GO:0032154 IEA
    protein-containing complex GO:0032991 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISS
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ppp1cc in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp1cc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cdk4  
Affinity Capture-Western Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Cdc34  
Affinity Capture-Western Mus musculus
4 Gsk3a  
Affinity Capture-Western Mus musculus
5 Wbp11  
Reconstituted Complex Mus musculus
6 Cdk1 12534
Affinity Capture-Western Mus musculus
7 Ccnb1  
Affinity Capture-Western Mus musculus
8 Bcl2  
Affinity Capture-Western Mus musculus
9 Eif2a  
Affinity Capture-Western Mus musculus
10 Pou5f1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
11 Ccnd1  
Affinity Capture-Western Mus musculus
12 Stx1a  
Affinity Capture-Western Mus musculus
13 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Dlg3  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
15 Skp1a 21402
Affinity Capture-Western Mus musculus
16 Cdk2  
Affinity Capture-Western Mus musculus
17 Trp53  
Affinity Capture-Western Mus musculus
18 Mapt  
Affinity Capture-MS Mus musculus
19 Atxn1  
Affinity Capture-MS Mus musculus
20 Kctd13  
Affinity Capture-MS Mus musculus
21 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
22 Ccnd3  
Affinity Capture-Western Mus musculus
23 Ywhaz 22631
Affinity Capture-MS Mus musculus
24 Max  
Affinity Capture-Western Mus musculus
25 Mettl21e  
Affinity Capture-MS Mus musculus
26 Tmem173  
Proximity Label-MS Mus musculus
27 Grin2b  
Affinity Capture-Western Mus musculus
28 Cyfip1 20430
Affinity Capture-MS Mus musculus
29 Cdkn2a  
Affinity Capture-Western Mus musculus
30 Rb1  
Affinity Capture-Western Mus musculus
31 Twist1  
Proximity Label-MS Mus musculus
32 Grin1  
Affinity Capture-Western Mus musculus
33 Hspa1b 15511
Affinity Capture-Western Mus musculus
34 Zbtb7b  
Affinity Capture-MS Mus musculus
35 Pcna 18538
Affinity Capture-Western Mus musculus
36 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
37 Eed  
Affinity Capture-MS Mus musculus
38 Egfr 13649
Affinity Capture-Western Mus musculus
39 Ppp1cb 19046
Co-fractionation Mus musculus
40 Atg16l1  
Affinity Capture-MS Mus musculus
41 PRKACB  
Affinity Capture-MS Bos taurus
42 Tsg101 22088
Affinity Capture-Western Mus musculus
43 Bcl2l1  
Affinity Capture-Western Mus musculus
44 Esr1  
Affinity Capture-Western Mus musculus
45 Bax 12028
Affinity Capture-Western Mus musculus
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