Gene description for PIKFYVE
Gene name phosphoinositide kinase, FYVE finger containing
Gene symbol PIKFYVE
Other names/aliases CFD
FAB1
HEL37
PIP5K
PIP5K3
ZFYVE29
Species Homo sapiens
 Database cross references - PIKFYVE
ExoCarta ExoCarta_200576
Vesiclepedia VP_200576
Entrez Gene 200576
HGNC 23785
MIM 609414
UniProt Q9Y2I7  
 PIKFYVE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PIKFYVE
Molecular Function
    1-phosphatidylinositol-3-phosphate 5-kinase activity GO:0000285 IBA
    1-phosphatidylinositol-3-phosphate 5-kinase activity GO:0000285 IDA
    1-phosphatidylinositol-3-phosphate 5-kinase activity GO:0000285 ISS
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IEA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 TAS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity GO:0043813 TAS
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    1-phosphatidylinositol-5-kinase activity GO:0052810 IBA
    1-phosphatidylinositol-5-kinase activity GO:0052810 IDA
    1-phosphatidylinositol-5-kinase activity GO:0052810 ISS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein targeting to membrane GO:0006612 ISS
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    receptor-mediated endocytosis GO:0006898 IMP
    receptor-mediated endocytosis of virus by host cell GO:0019065 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    neutrophil chemotaxis GO:0030593 IBA
    neutrophil chemotaxis GO:0030593 IDA
    myelin assembly GO:0032288 IEA
    melanosome organization GO:0032438 IBA
    melanosome organization GO:0032438 IDA
    protein localization to nucleus GO:0034504 IMP
    intracellular signal transduction GO:0035556 IEA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IEA
    peptidyl-serine autophosphorylation GO:0036289 ISS
    retrograde transport, endosome to Golgi GO:0042147 IMP
    phagosome maturation GO:0090382 IDA
    phagosome-lysosome fusion GO:0090385 IBA
    phagosome-lysosome fusion GO:0090385 IDA
    1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate metabolic process GO:1903100 ISS
    regulation of reactive oxygen species biosynthetic process GO:1903426 IBA
    regulation of reactive oxygen species biosynthetic process GO:1903426 IDA
    phosphatidylinositol 5-phosphate metabolic process GO:1904562 IC
    phosphatidylinositol 5-phosphate metabolic process GO:1904562 ISS
    regulation of autophagosome assembly GO:2000785 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    cytosol GO:0005829 IEA
    cell-cell junction GO:0005911 IEA
    endosome membrane GO:0010008 IDA
    vesicle membrane GO:0012506 IBA
    phagocytic vesicle membrane GO:0030670 IDA
    cytoplasmic vesicle GO:0031410 IBA
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 TAS
    late endosome membrane GO:0031902 TAS
    membrane raft GO:0045121 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PIKFYVE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PIKFYVE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FIG4 9896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GTPBP3  
Affinity Capture-MS Homo sapiens
3 RABEPK  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
4 LAMTOR1 55004
Proximity Label-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 RAB5A 5868
Proximity Label-MS Homo sapiens
7 VAC14 55697
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 ATG9A 79065
Proximity Label-MS Homo sapiens
9 NPTN 27020
Affinity Capture-MS Homo sapiens
10 ANK1 286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LCAT 3931
Co-crystal Structure Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 CDK5 1020
Biochemical Activity Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 OCIAD1 54940
Proximity Label-MS Homo sapiens
16 RAB4A 5867
Proximity Label-MS Homo sapiens
17 CAPZB 832
Affinity Capture-MS Homo sapiens
18 NTRK1 4914
Affinity Capture-MS Homo sapiens
19 ESD 2098
Affinity Capture-MS Homo sapiens
20 RAB7A 7879
Proximity Label-MS Homo sapiens
21 RAB9A 9367
Proximity Label-MS Homo sapiens
22 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TIMM23 100287932
Affinity Capture-MS Homo sapiens
24 BCL6  
Affinity Capture-MS Homo sapiens
25 KRAS 3845
Negative Genetic Homo sapiens
26 PTCH1  
Affinity Capture-MS Homo sapiens
27 PTAR1 375743
Synthetic Lethality Homo sapiens
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