Gene description for WDR81
Gene name WD repeat domain 81
Gene symbol WDR81
Other names/aliases CAMRQ2
PPP1R166
Species Homo sapiens
 Database cross references - WDR81
ExoCarta ExoCarta_124997
Vesiclepedia VP_124997
Entrez Gene 124997
HGNC 26600
MIM 614218
UniProt Q562E7  
 WDR81 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for WDR81
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol 3-kinase regulator activity GO:0035014 IBA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IDA
    phosphatidylinositol 3-kinase inhibitor activity GO:0141039 IMP
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    mitochondrion organization GO:0007005 ISS
    aggrephagy GO:0035973 IBA
    aggrephagy GO:0035973 IMP
    early endosome to late endosome transport GO:0045022 IMP
    protein stabilization GO:0050821 IMP
Subcellular Localization
    autophagosome membrane GO:0000421 IDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    lysosomal membrane GO:0005765 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    endosome membrane GO:0010008 IDA
    early endosome membrane GO:0031901 IDA
    late endosome membrane GO:0031902 IDA
 Experiment description of studies that identified WDR81 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDR81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP4C 5531
Affinity Capture-MS Homo sapiens
2 NPAS1  
Affinity Capture-MS Homo sapiens
3 XPO1 7514
Affinity Capture-MS Homo sapiens
4 NAPA 8775
Affinity Capture-MS Homo sapiens
5 CCT2 10576
Affinity Capture-MS Homo sapiens
6 CUL3 8452
Affinity Capture-MS Homo sapiens
7 DCAF13 25879
Affinity Capture-MS Homo sapiens
8 LAMP2 3920
Proximity Label-MS Homo sapiens
9 WFDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 METTL21B  
Affinity Capture-MS Homo sapiens
11 DDX58 23586
Affinity Capture-RNA Homo sapiens
12 NXF1 10482
Affinity Capture-RNA Homo sapiens
13 C12orf74  
Affinity Capture-MS Homo sapiens
14 IFI35 3430
Affinity Capture-MS Homo sapiens
15 MOV10 4343
Affinity Capture-RNA Homo sapiens
16 INF2 64423
Affinity Capture-MS Homo sapiens
17 NTRK1 4914
Affinity Capture-MS Homo sapiens
18 RAB7A 7879
Proximity Label-MS Homo sapiens
19 SHMT2 6472
Affinity Capture-RNA Homo sapiens
20 F12  
Affinity Capture-MS Homo sapiens
21 ENO1 2023
Affinity Capture-RNA Homo sapiens
22 SF3B3 23450
Affinity Capture-MS Homo sapiens
23 BLNK  
Affinity Capture-MS Homo sapiens
24 MAPK15  
Affinity Capture-MS Homo sapiens
25 UBXN6 80700
Affinity Capture-MS Homo sapiens
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