Experiment description of studies that identified SHANK3 in exosomes

1 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

2 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for SHANK3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MAPK1 5594 Two-hybrid Homo sapiens 2 NCK1 4690 Two-hybrid Homo sapiens 3 ARHGEF7 8874 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 4 BTF3 689 Affinity Capture-MS Homo sapiens 5 PRMT2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 ABCC2 1244 Two-hybrid Homo sapiens 7 PPP1CC 5501 Two-hybrid Homo sapiens 8 COPS5 10987 Two-hybrid Homo sapiens 9 NUMA1 4926 Affinity Capture-MS Homo sapiens 10 ARHGAP12   Affinity Capture-MS Homo sapiens 11 DLGAP3   Two-hybrid Homo sapiens 12 DLGAP1   Two-hybrid Homo sapiens 13 SHANK1   Affinity Capture-Western Homo sapiens 14 SPTAN1 6709 Two-hybrid Homo sapiens 15 CSNK2B 1460 Two-hybrid Homo sapiens 16 TP53 7157 Affinity Capture-MS Homo sapiens 17 CAMK2A 815 Affinity Capture-Western Homo sapiens 18 GRB2 2885 Two-hybrid Homo sapiens 19 CRKL 1399 Two-hybrid Homo sapiens 20 DLGAP4 22839 Two-hybrid Homo sapiens 21 DLGAP2   Two-hybrid Homo sapiens View the network

Pathways in which SHANK3 is involved

No pathways found