Gene description for ACOT8
Gene name acyl-CoA thioesterase 8
Gene symbol ACOT8
Other names/aliases HNAACTE
PTE-1
PTE-2
PTE1
PTE2
hACTE-III
hTE
Species Homo sapiens
 Database cross references - ACOT8
ExoCarta ExoCarta_10005
Vesiclepedia VP_10005
Entrez Gene 10005
HGNC 15919
MIM 608123
UniProt O14734  
 ACOT8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ACOT8
Molecular Function
    acetyl-CoA hydrolase activity GO:0003986 TAS
    succinyl-CoA hydrolase activity GO:0004778 IEA
    protein binding GO:0005515 IPI
    acyl-CoA hydrolase activity GO:0016289 TAS
    choloyl-CoA hydrolase activity GO:0033882 IEA
    acetoacetyl-CoA hydrolase activity GO:0047603 IEA
    fatty acyl-CoA hydrolase activity GO:0047617 IBA
    fatty acyl-CoA hydrolase activity GO:0047617 IDA
    fatty acyl-CoA hydrolase activity GO:0047617 TAS
    hydroxymethylglutaryl-CoA hydrolase activity GO:0047994 IEA
    carboxylic ester hydrolase activity GO:0052689 IEA
    medium-chain fatty acyl-CoA hydrolase activity GO:0052815 IDA
    long-chain fatty acyl-CoA hydrolase activity GO:0052816 IDA
    long-chain fatty acyl-CoA hydrolase activity GO:0052816 IDA
Biological Process
    acyl-CoA metabolic process GO:0006637 IBA
    acyl-CoA metabolic process GO:0006637 IDA
    acyl-CoA metabolic process GO:0006637 TAS
    bile acid biosynthetic process GO:0006699 TAS
    fatty acid catabolic process GO:0009062 IBA
    peroxisome fission GO:0016559 IDA
    fatty acid beta-oxidation using acyl-CoA oxidase GO:0033540 TAS
    alpha-linolenic acid metabolic process GO:0036109 TAS
    dicarboxylic acid catabolic process GO:0043649 IDA
    negative regulation of CD4 production GO:0045225 IDA
Subcellular Localization
    peroxisomal matrix GO:0005782 IBA
    peroxisomal matrix GO:0005782 IDA
    peroxisomal matrix GO:0005782 IMP
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ACOT8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ACOT8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PDHA1 5160
Affinity Capture-MS Homo sapiens
3 CDC26 246184
Affinity Capture-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 CUL3 8452
Affinity Capture-MS Homo sapiens
7 IGHA1 3493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PTPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 TRIM66  
Affinity Capture-MS Homo sapiens
11 BRD8 10902
Affinity Capture-MS Homo sapiens
12 MTMR14 64419
Affinity Capture-MS Homo sapiens
13 NR3C1 2908
Proximity Label-MS Homo sapiens
14 PTP4A1 7803
Affinity Capture-MS Homo sapiens
15 VSTM1  
Affinity Capture-MS Homo sapiens
16 KLHL23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SNX21  
Affinity Capture-MS Homo sapiens
18 TAF1  
Affinity Capture-MS Homo sapiens
19 PRDM16 63976
Two-hybrid Homo sapiens
20 MYC  
Affinity Capture-MS Homo sapiens
21 CIT 11113
Affinity Capture-MS Homo sapiens
22 BRD1 23774
Affinity Capture-MS Homo sapiens
23 PEX5 5830
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
24 COA7  
Affinity Capture-MS Homo sapiens
25 RNF2  
Affinity Capture-MS Homo sapiens
26 C9orf72  
Affinity Capture-MS Homo sapiens
27 Nedd1  
Affinity Capture-MS Mus musculus
28 P4HA3 283208
Two-hybrid Homo sapiens
29 PML 5371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 AGPS 8540
Proximity Label-MS Homo sapiens
31 SP100 6672
Affinity Capture-MS Homo sapiens
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