Gene description for LPGAT1
Gene name lysophosphatidylglycerol acyltransferase 1
Gene symbol LPGAT1
Other names/aliases FAM34A
FAM34A1
NET8
Species Homo sapiens
 Database cross references - LPGAT1
ExoCarta ExoCarta_9926
Vesiclepedia VP_9926
Entrez Gene 9926
HGNC 28985
MIM 610473
UniProt Q92604  
 LPGAT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for LPGAT1
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    2-acylglycerol O-acyltransferase activity GO:0003846 ISS
    acyltransferase activity GO:0016746 IBA
    2-acylglycerol-3-phosphate O-acyltransferase activity GO:0047144 TAS
    2-acylglycerophosphocholine O-acyltransferase activity GO:0047190 IDA
    lysophospholipid acyltransferase activity GO:0071617 IDA
    lysophospholipid acyltransferase activity GO:0071617 IMP
    lysophosphatidylethanolamine acyltransferase activity GO:0071618 ISS
Biological Process
    phospholipid biosynthetic process GO:0008654 IEA
    triglyceride biosynthetic process GO:0019432 ISS
    phosphatidylglycerol acyl-chain remodeling GO:0036148 TAS
    phosphatidylinositol acyl-chain remodeling GO:0036149 IBA
    phosphatidylethanolamine acyl-chain remodeling GO:0036152 ISS
    positive regulation of fatty acid biosynthetic process GO:0045723 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IMP
    endoplasmic reticulum membrane GO:0005789 TAS
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified LPGAT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for LPGAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 HSPA1L 3305
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 PSMB10 5699
Affinity Capture-MS Homo sapiens
7 IZUMO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 VAPA 9218
Affinity Capture-MS Homo sapiens
9 FURIN 5045
Affinity Capture-MS Homo sapiens
10 CANX 821
Affinity Capture-MS Homo sapiens
11 ATL3 25923
Affinity Capture-MS Homo sapiens
12 TMEM184A  
Affinity Capture-MS Homo sapiens
13 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HLA-B 3106
Affinity Capture-MS Homo sapiens
15 KCNK1  
Affinity Capture-MS Homo sapiens
16 Bmpr1a  
Affinity Capture-MS Mus musculus
17 RNF41  
Affinity Capture-MS Homo sapiens
18 BCAP31 10134
Proximity Label-MS Homo sapiens
19 TSPAN15 23555
Affinity Capture-MS Homo sapiens
20 FPR1  
Affinity Capture-MS Homo sapiens
21 MBOAT1  
Affinity Capture-MS Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
24 UNC93B1 81622
Affinity Capture-MS Homo sapiens
25 AQP3  
Affinity Capture-MS Homo sapiens
26 OPRL1 4987
Affinity Capture-MS Homo sapiens
27 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
28 FFAR1  
Affinity Capture-MS Homo sapiens
29 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 VSIG4  
Affinity Capture-MS Homo sapiens
31 KIAA1429 25962
Affinity Capture-MS Homo sapiens
32 PLD3 23646
Affinity Capture-MS Homo sapiens
33 B3GAT1  
Proximity Label-MS Homo sapiens
34 TUBA1C 84790
Affinity Capture-MS Homo sapiens
35 SEC62 7095
Proximity Label-MS Homo sapiens
36 KLRG2 346689
Affinity Capture-MS Homo sapiens
37 GPR45  
Affinity Capture-MS Homo sapiens
38 C3orf52  
Affinity Capture-MS Homo sapiens
39 CHODL 140578
Affinity Capture-MS Homo sapiens
40 Hax1  
Affinity Capture-MS Mus musculus
41 GPR182  
Affinity Capture-MS Homo sapiens
42 SLC2A5 6518
Affinity Capture-MS Homo sapiens
43 CHST15 51363
Affinity Capture-MS Homo sapiens
44 LYPD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 VIPR1  
Affinity Capture-MS Homo sapiens
46 GPR55  
Affinity Capture-MS Homo sapiens
47 Rpl35 66489
Affinity Capture-MS Mus musculus
48 GPBAR1  
Affinity Capture-MS Homo sapiens
49 SLC1A1 6505
Affinity Capture-MS Homo sapiens
50 P2RY8  
Affinity Capture-MS Homo sapiens
51 RPA3 6119
Proximity Label-MS Homo sapiens
52 FPR2  
Affinity Capture-MS Homo sapiens
53 SEC61B 10952
Proximity Label-MS Homo sapiens
54 SGCZ  
Affinity Capture-MS Homo sapiens
55 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PCDHB3  
Affinity Capture-MS Homo sapiens
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