Gene description for ALKBH5
Gene name AlkB family member 5, RNA demethylase
Gene symbol ALKBH5
Other names/aliases ABH5
OFOXD
OFOXD1
Species Homo sapiens
 Database cross references - ALKBH5
ExoCarta ExoCarta_54890
Vesiclepedia VP_54890
Entrez Gene 54890
HGNC 25996
MIM 613303
UniProt Q6P6C2  
 ALKBH5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ALKBH5
Molecular Function
    RNA binding GO:0003723 HDA
    2-oxoglutarate-dependent dioxygenase activity GO:0016706 IDA
    oxidative RNA demethylase activity GO:0035515 IBA
    metal ion binding GO:0046872 IEA
    molecular condensate scaffold activity GO:0140693 IDA
    mRNA N6-methyladenosine dioxygenase activity GO:1990931 IDA
Biological Process
    response to hypoxia GO:0001666 IDA
    mRNA processing GO:0006397 IEA
    regulation of translation GO:0006417 IDA
    spermatogenesis GO:0007283 ISS
    regulation of mRNA export from nucleus GO:0010793 IMP
    cell differentiation GO:0030154 IEA
    gamma-delta T cell proliferation GO:0046630 IEA
    regulation of mRNA processing GO:0050684 IMP
    mRNA destabilization GO:0061157 IDA
    mRNA destabilization GO:0061157 ISS
    membraneless organelle assembly GO:0140694 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IDA
    paraspeckles GO:0042382 IDA
 Experiment description of studies that identified ALKBH5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALKBH5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 MINK1 50488
Affinity Capture-MS Homo sapiens
4 PRPF4B 8899
Affinity Capture-MS Homo sapiens
5 RBM8A 9939
Affinity Capture-MS Homo sapiens
6 KPNA1 3836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ADSL 158
Co-fractionation Homo sapiens
8 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
9 EPB41L5 57669
Affinity Capture-MS Homo sapiens
10 KPNA6 23633
Affinity Capture-MS Homo sapiens
11 LMNA 4000
Proximity Label-MS Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
14 C17orf85  
Proximity Label-MS Homo sapiens
15 A2M 2
Co-fractionation Homo sapiens
16 HECW2  
Affinity Capture-MS Homo sapiens
17 HMGN1  
Cross-Linking-MS (XL-MS) Homo sapiens
18 OGT 8473
Reconstituted Complex Homo sapiens
19 PAPSS1 9061
Co-fractionation Homo sapiens
20 NAGA 4668
Co-fractionation Homo sapiens
21 DDRGK1 65992
Affinity Capture-MS Homo sapiens
22 DDX39B 7919
Affinity Capture-MS Homo sapiens
23 CEBPA  
Protein-peptide Homo sapiens
24 THOC7 80145
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ALKBH5 is involved
PathwayEvidenceSource
DNA Damage Reversal TAS Reactome
DNA Repair TAS Reactome
Reversal of alkylation damage by DNA dioxygenases TAS Reactome





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