Gene description for PKD1
Gene name polycystic kidney disease 1 (autosomal dominant)
Gene symbol PKD1
Other names/aliases PBP
Pc-1
TRPP1
Species Homo sapiens
 Database cross references - PKD1
ExoCarta ExoCarta_5310
Entrez Gene 5310
HGNC 9008
MIM 601313
UniProt P98161  
 PKD1 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for PKD1
Molecular Function
    ion channel binding GO:0044325 IPI
    carbohydrate binding GO:0030246 IEA
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    calcium channel activity GO:0005262 ISS
Biological Process
    mesonephric tubule development GO:0072164 IEP
    neural tube development GO:0021915 IEP
    kidney development GO:0001822 ISS
    mesonephric duct development GO:0072177 IEP
    placenta blood vessel development GO:0060674 ISS
    peptidyl-serine phosphorylation GO:0018105 ISS
    metanephric proximal tubule development GO:0072237 IEP
    liver development GO:0001889 IEA
    calcium-independent cell-matrix adhesion GO:0007161 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    metanephric collecting duct development GO:0072205 IEP
    positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle GO:0031659 IDA
    organelle organization GO:0006996 TAS
    cell-matrix adhesion GO:0007160 TAS
    branching morphogenesis of an epithelial tube GO:0048754 IDA
    detection of mechanical stimulus GO:0050982 ISS
    positive regulation of protein binding GO:0032092 ISS
    anatomical structure morphogenesis GO:0009653 TAS
    JAK-STAT cascade GO:0007259 ISS
    cartilage development GO:0051216 IEP
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    nitrogen compound metabolic process GO:0006807 IEA
    protein export from nucleus GO:0006611 ISS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 TAS
    digestive tract development GO:0048565 IEP
    genitalia development GO:0048806 IEP
    embryonic placenta development GO:0001892 ISS
    metanephric ascending thin limb development GO:0072218 IEP
    skin development GO:0043588 IEP
    calcium ion transmembrane transport GO:0070588 ISS
    response to fluid shear stress GO:0034405 IEA
    regulation of proteasomal protein catabolic process GO:0061136 IDA
    spinal cord development GO:0021510 IEP
    cell cycle arrest GO:0007050 ISS
    cytoplasmic sequestering of transcription factor GO:0042994 ISS
    in utero embryonic development GO:0001701 ISS
    regulation of mitotic spindle organization GO:0060236 IEA
    cartilage condensation GO:0001502 IEA
    single organismal cell-cell adhesion GO:0016337 IEA
    heart development GO:0007507 IEP
    lung epithelium development GO:0060428 IEP
    metanephric distal tubule morphogenesis GO:0072287 IEP
Subcellular Localization
    basolateral plasma membrane GO:0016323 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    integral component of membrane GO:0016021 NAS
    polycystin complex GO:0002133 ISS
    Golgi membrane GO:0000139 TAS
    lateral plasma membrane GO:0016328 IEA
    motile primary cilium GO:0031512 ISS
    cilium GO:0005929 ISS
    primary cilium GO:0072372 ISS
    integral component of plasma membrane GO:0005887 TAS
    Golgi-associated vesicle membrane GO:0030660 TAS
    nucleus GO:0005634 ISS
    ciliary membrane GO:0060170 TAS
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PKD1 in exosomes
1
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PKD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FN1 2335
Reconstituted Complex Homo sapiens
2 RGS7  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 PKD2 5311
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 COL1A1 1277
Reconstituted Complex Homo sapiens
5 CTNNB1 1499
Invitro Homo sapiens
Invivo Homo sapiens
6 CDH1 999
Invivo Homo sapiens
Invitro Homo sapiens
7 JUP 3728
Invivo Homo sapiens
Invitro Homo sapiens
8 KRT18 3875
Reconstituted Complex Homo sapiens
9 COL2A1 1280
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which PKD1 is involved
PathwayEvidenceSource
VxPx cargo-targeting to cilium TAS Reactome





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