Gene description for PGK2
Gene name phosphoglycerate kinase 2
Gene symbol PGK2
Other names/aliases HEL-S-272
PGKB
PGKPS
dJ417L20.2
Species Homo sapiens
 Database cross references - PGK2
ExoCarta ExoCarta_5232
Vesiclepedia VP_5232
Entrez Gene 5232
HGNC 8898
MIM 172270
UniProt P07205  
 PGK2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for PGK2
Molecular Function
    phosphoglycerate kinase activity GO:0004618 EXP
    phosphoglycerate kinase activity GO:0004618 IBA
    phosphoglycerate kinase activity GO:0004618 ISS
    phosphoglycerate kinase activity GO:0004618 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 ISS
    ADP binding GO:0043531 IBA
Biological Process
    gluconeogenesis GO:0006094 IBA
    gluconeogenesis GO:0006094 TAS
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 NAS
    phosphorylation GO:0016310 NAS
    flagellated sperm motility GO:0030317 IEA
    canonical glycolysis GO:0061621 TAS
Subcellular Localization
    nucleus GO:0005634 HDA
    cytosol GO:0005829 IBA
    sperm fibrous sheath GO:0035686 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PGK2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PGK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENO3 2027
Co-fractionation Homo sapiens
2 UTP15 84135
Affinity Capture-MS Homo sapiens
3 HSPE1 3336
Co-fractionation Homo sapiens
4 LDHAL6A  
Co-fractionation Homo sapiens
5 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
6 IP6K2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ALDOC 230
Co-fractionation Homo sapiens
8 SCMH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 AIFM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ORM1 5004
Affinity Capture-MS Homo sapiens
11 C7orf31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SMNDC1  
Cross-Linking-MS (XL-MS) Homo sapiens
13 INTS10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 POF1B 79983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SENP2 59343
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ZSCAN12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
18 SHMT1 6470
Co-fractionation Homo sapiens
19 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FLCN 201163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 AKR1B1 231
Co-fractionation Homo sapiens
22 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PRDM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RPE  
Co-fractionation Homo sapiens
25 ABTB2 25841
Affinity Capture-MS Homo sapiens
26 SYT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PGK1 5230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SLC13A2 9058
Affinity Capture-MS Homo sapiens
29 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
30 HOXC4  
Affinity Capture-MS Homo sapiens
31 HEPN1  
Affinity Capture-MS Homo sapiens
32 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 POLL  
Affinity Capture-MS Homo sapiens
34 ENO1 2023
Co-fractionation Homo sapiens
35 ZNF460  
Affinity Capture-MS Homo sapiens
36 RNF13 11342
Affinity Capture-MS Homo sapiens
37 MSN 4478
Co-fractionation Homo sapiens
38 LRRC40 55631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 GDPD1 284161
Affinity Capture-MS Homo sapiens
40 AKR1A1 10327
Co-fractionation Homo sapiens
41 ASPA  
Affinity Capture-MS Homo sapiens
42 ZCCHC12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ZNF416  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TAGLN3 29114
Co-fractionation Homo sapiens
45 FAM71D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PGK2 is involved
PathwayEvidenceSource
Gluconeogenesis TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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