Gene description for ARHGEF3
Gene name Rho guanine nucleotide exchange factor (GEF) 3
Gene symbol ARHGEF3
Other names/aliases GEF3
STA3
XPLN
Species Homo sapiens
 Database cross references - ARHGEF3
ExoCarta ExoCarta_50650
Entrez Gene 50650
HGNC 683
MIM 612115
UniProt Q9NR81  
 ARHGEF3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ARHGEF3
Molecular Function
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    protein binding GO:0005515 IPI
Biological Process
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    apoptotic signaling pathway GO:0097190 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    Rho protein signal transduction GO:0007266 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    intracellular GO:0005622 IDA
 Experiment description of studies that identified ARHGEF3 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ARHGEF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOA 387
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
2 RHOB 388
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which ARHGEF3 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events TAS Reactome
NRAGE signals death through JNK TAS Reactome
Rho GTPase cycle TAS Reactome





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