Gene description for APCS
Gene name amyloid P component, serum
Gene symbol APCS
Other names/aliases HEL-S-92n
PTX2
SAP
Species Homo sapiens
 Database cross references - APCS
ExoCarta ExoCarta_325
Entrez Gene 325
HGNC 584
MIM 104770
UniProt P02743  
 APCS identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for APCS
Molecular Function
    virion binding GO:0046790 IDA
    carbohydrate binding GO:0030246 IEA
    complement component C1q binding GO:0001849 IDA
    calcium ion binding GO:0005509 IDA
    unfolded protein binding GO:0051082 TAS
Biological Process
    negative regulation by host of viral glycoprotein metabolic process GO:0044871 IDA
    negative regulation of acute inflammatory response GO:0002674 IC
    negative regulation of monocyte differentiation GO:0045656 IDA
    negative regulation of wound healing GO:0061045 IC
    chaperone-mediated protein complex assembly GO:0051131 TAS
    negative regulation of glycoprotein metabolic process GO:1903019 IDA
    acute-phase response GO:0006953 TAS
    negative regulation of exo-alpha-sialidase activity GO:1903016 IDA
    innate immune response GO:0045087 IDA
    protein folding GO:0006457 TAS
    negative regulation of viral entry into host cell GO:0046597 IDA
    negative regulation by host of viral exo-alpha-sialidase activity GO:0044869 IDA
    negative regulation of viral process GO:0048525 IDA
Subcellular Localization
    extracellular matrix GO:0031012 IDA
    protein complex GO:0043234 IEA
    blood microparticle GO:0072562 IDA
    extracellular space GO:0005615 IDA
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified APCS in exosomes
1
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for APCS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FN1 2335
Invivo Homo sapiens
2 APCS 325
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
3 C1QA 712
Invitro Homo sapiens
4 FCGR1A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 TG  
Invitro Homo sapiens
6 FCGR3A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 CALU 813
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
8 FCGR3B  
Reconstituted Complex Homo sapiens
9 C4BPA 722
Co-purification Homo sapiens
10 FCGR2B  
Affinity Capture-MS Homo sapiens
11 CRP  
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which APCS is involved
PathwayEvidenceSource
Amyloids TAS Reactome





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