Gene description for FBXO9
Gene name F-box protein 9
Gene symbol FBXO9
Other names/aliases FBX9
NY-REN-57
VCIA1
dJ341E18.2
Species Homo sapiens
 Database cross references - FBXO9
ExoCarta ExoCarta_26268
Vesiclepedia VP_26268
Entrez Gene 26268
HGNC 13588
MIM 609091
UniProt Q9UK97  
 FBXO9 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FBXO9
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 NAS
    protein binding GO:0005515 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 NAS
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IDA
    regulation of TOR signaling GO:0032006 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    innate immune response GO:0045087 IEA
    fat cell differentiation GO:0045444 IEA
    protein K48-linked ubiquitination GO:0070936 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 NAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IBA
    SCF ubiquitin ligase complex GO:0019005 IDA
 Experiment description of studies that identified FBXO9 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FBXO9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPS5 10987
Affinity Capture-MS Homo sapiens
2 Skp1a 21402
Affinity Capture-MS Mus musculus
3 Pparg  
Affinity Capture-MS Mus musculus
4 EEF2 1938
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 S100P 6286
Affinity Capture-MS Homo sapiens
8 CARM1 10498
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 RBX1 9978
Affinity Capture-MS Homo sapiens
10 CALM1 801
Reconstituted Complex Homo sapiens
11 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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