Gene description for Stx4a
Gene name syntaxin 4A (placental)
Gene symbol Stx4a
Other names/aliases Stx4
Syn-4
Syn4
Species Mus musculus
 Database cross references - Stx4a
ExoCarta ExoCarta_20909
Vesiclepedia VP_20909
Entrez Gene 20909
UniProt P70452  
 Stx4a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stx4a
Molecular Function
    SNARE binding GO:0000149 IBA
    SNARE binding GO:0000149 IPI
    SNARE binding GO:0000149 ISO
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IEA
    SNAP receptor activity GO:0005484 ISO
    protein binding GO:0005515 IPI
    sphingomyelin phosphodiesterase activator activity GO:0016230 IEA
    sphingomyelin phosphodiesterase activator activity GO:0016230 ISO
    small GTPase binding GO:0031267 ISO
Biological Process
    positive regulation of immunoglobulin production GO:0002639 IEA
    positive regulation of immunoglobulin production GO:0002639 ISO
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    exocytosis GO:0006887 IBA
    vesicle fusion GO:0006906 IBA
    sensory perception of sound GO:0007605 ISS
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    synaptic vesicle exocytosis GO:0016079 TAS
    synaptic vesicle docking GO:0016081 ISO
    regulation of exocytosis GO:0017157 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    protein localization to cell surface GO:0034394 ISO
    protein localization to cell surface GO:0034394 ISS
    cellular response to oxidative stress GO:0034599 IEA
    cellular response to oxidative stress GO:0034599 ISO
    SNARE complex assembly GO:0035493 IDA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IEA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    positive regulation of catalytic activity GO:0043085 ISO
    positive regulation of eosinophil degranulation GO:0043311 IEA
    positive regulation of eosinophil degranulation GO:0043311 ISO
    positive regulation of cell adhesion GO:0045785 IEA
    positive regulation of cell adhesion GO:0045785 ISO
    vesicle docking GO:0048278 IBA
    organelle fusion GO:0048284 ISO
    positive regulation of chemotaxis GO:0050921 IEA
    positive regulation of chemotaxis GO:0050921 ISO
    long-term synaptic potentiation GO:0060291 IEA
    long-term synaptic potentiation GO:0060291 ISO
    membrane fusion GO:0061025 ISO
    protein-containing complex assembly GO:0065003 ISO
    cellular response to type II interferon GO:0071346 IDA
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 ISO
    vesicle-mediated transport in synapse GO:0099003 ISO
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IDA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IMP
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    cornified envelope assembly GO:1903575 IEA
    cornified envelope assembly GO:1903575 ISO
    positive regulation of protein localization to cell surface GO:2000010 IEA
    positive regulation of protein localization to cell surface GO:2000010 ISO
Subcellular Localization
    storage vacuole GO:0000322 IDA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    trans-Golgi network GO:0005802 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IPI
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    SNARE complex GO:0031201 ISO
    SNARE complex GO:0031201 ISS
    stereocilium GO:0032420 IDA
    neuron projection membrane GO:0032589 ISO
    neuron projection membrane GO:0032589 ISS
    myelin sheath adaxonal region GO:0035749 IDA
    somatodendritic compartment GO:0036477 ISO
    sarcolemma GO:0042383 ISO
    specific granule GO:0042581 IEA
    specific granule GO:0042581 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    lateral loop GO:0043219 IDA
    synapse GO:0045202 ISO
    postsynaptic membrane GO:0045211 ISO
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Stx4a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stx4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stx6  
Co-fractionation Mus musculus
2 Stx3  
Co-fractionation Mus musculus
3 Vamp5  
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Vamp4  
Co-fractionation Mus musculus
6 Snap29  
Co-fractionation Mus musculus
7 Runx2  
Affinity Capture-MS Mus musculus
8 Stx7  
Affinity Capture-Western Mus musculus
9 Stxbp4  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
10 Snap23 20619
Co-fractionation Mus musculus
11 Bnip1  
Co-fractionation Mus musculus
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