Gene description for Atp1a1
Gene name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Gene symbol Atp1a1
Other names/aliases Atpa-1
BC010319
Species Mus musculus
 Database cross references - Atp1a1
ExoCarta ExoCarta_11928
Entrez Gene 11928
UniProt Q8VDN2  
 Atp1a1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Atp1a1
Molecular Function
    nucleotide binding GO:0000166 IEA
    ankyrin binding GO:0030506 ISO
    ATP binding GO:0005524 ISO
    ADP binding GO:0043531 ISO
    phosphatase activity GO:0016791 IMP
    protein domain specific binding GO:0019904 ISO
    phosphatidylinositol 3-kinase binding GO:0043548 ISO
    sodium:potassium-exchanging ATPase activity GO:0005391 ISO
    sodium ion binding GO:0031402 ISO
    protein kinase binding GO:0019901 ISO
    protein binding GO:0005515 IPI
    steroid hormone binding GO:1990239 ISO
    chaperone binding GO:0051087 ISO
    hydrolase activity GO:0016787 IEA
    metal ion binding GO:0046872 IEA
    potassium ion binding GO:0030955 ISO
Biological Process
    sodium ion transmembrane transport GO:0035725 IMP
    cellular sodium ion homeostasis GO:0006883 ISO
    response to glycoside GO:1903416 IGI
    regulation of sodium ion transport GO:0002028 ISO
    potassium ion transport GO:0006813 ISO
    negative regulation of heart contraction GO:0045822 IMP
    regulation of the force of heart contraction GO:0002026 IMP
    dephosphorylation GO:0016311 IMP
    sodium ion transport GO:0006814 ISO
    potassium ion transmembrane transport GO:0071805 IMP
    cellular response to steroid hormone stimulus GO:0071383 ISO
    membrane hyperpolarization GO:0060081 ISO
    cellular response to mechanical stimulus GO:0071260 ISO
    cellular potassium ion homeostasis GO:0030007 ISO
    negative regulation of glucocorticoid biosynthetic process GO:0031947 IMP
    positive regulation of striated muscle contraction GO:0045989 IMP
    positive regulation of heart contraction GO:0045823 IMP
    regulation of blood pressure GO:0008217 IGI
    response to drug GO:0042493 IMP
    potassium ion import across plasma membrane GO:1990573 ISO
    potassium ion import GO:0010107 ISO
    transport GO:0006810 IEA
    ion transport GO:0006811 IEA
    membrane repolarization GO:0086009 ISO
    sodium ion export from cell GO:0036376 ISO
    regulation of cardiac muscle cell contraction GO:0086004 ISO
Subcellular Localization
    caveola GO:0005901 ISO
    basolateral plasma membrane GO:0016323 IDA
    neuronal postsynaptic density GO:0097481 IDA
    myelin sheath GO:0043209 IDA
    endoplasmic reticulum GO:0005783 ISO
    apical plasma membrane GO:0016324 IDA
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 ISO
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    sarcolemma GO:0042383 ISO
    membrane raft GO:0045121 ISO
    Golgi apparatus GO:0005794 ISO
    extracellular vesicle GO:1903561 ISO
    extracellular exosome GO:0070062 ISO
    endosome GO:0005768 ISO
    protein complex GO:0043234 ISO
    intercalated disc GO:0014704 ISO
    T-tubule GO:0030315 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Atp1a1 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Atp1a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp1a1 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases IEA Reactome





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