Gene description for PARD3B
Gene name par-3 family cell polarity regulator beta
Gene symbol PARD3B
Other names/aliases ALS2CR19
PAR3B
PAR3L
PAR3beta
Species Homo sapiens
 Database cross references - PARD3B
ExoCarta ExoCarta_117583
Vesiclepedia VP_117583
Entrez Gene 117583
HGNC 14446
UniProt Q8TEW8  
 PARD3B identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PARD3B
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IBA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    cell adhesion GO:0007155 IBA
    protein localization GO:0008104 IBA
    establishment of cell polarity GO:0030010 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    cell division GO:0051301 IEA
    establishment of centrosome localization GO:0051660 IBA
Subcellular Localization
    adherens junction GO:0005912 IBA
    bicellular tight junction GO:0005923 IEA
    cell cortex GO:0005938 IBA
    endomembrane system GO:0012505 IEA
    apical plasma membrane GO:0016324 IBA
    cell junction GO:0030054 IDA
    protein-containing complex GO:0032991 IDA
    apical junction complex GO:0043296 IBA
 Experiment description of studies that identified PARD3B in sEVs
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PARD3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 PPP3CA 5530
Affinity Capture-MS Homo sapiens
3 RBM5  
Two-hybrid Homo sapiens
4 CIB2  
Affinity Capture-MS Homo sapiens
5 CASK 8573
Two-hybrid Homo sapiens
6 PGP 283871
Affinity Capture-MS Homo sapiens
7 SNRPB 6628
Affinity Capture-MS Homo sapiens
8 PARD3 56288
Proximity Label-MS Homo sapiens
9 CTNNB1 1499
Proximity Label-MS Homo sapiens
10 TREX2  
Affinity Capture-MS Homo sapiens
11 GAA 2548
Two-hybrid Homo sapiens
12 SNRPF 6636
Affinity Capture-MS Homo sapiens
13 SNRPD2 6633
Affinity Capture-MS Homo sapiens
14 KLHL13 90293
Affinity Capture-MS Homo sapiens
15 MCAM 4162
Proximity Label-MS Homo sapiens
16 NPTXR 23467
Affinity Capture-MS Homo sapiens
17 CEP250 11190
Affinity Capture-MS Homo sapiens
18 MARK2 2011
Two-hybrid Homo sapiens
19 YWHAE 7531
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 PPP3CB  
Affinity Capture-MS Homo sapiens
21 EPHA2 1969
Proximity Label-MS Homo sapiens
22 BICD2 23299
Proximity Label-MS Homo sapiens
23 RHOB 388
Proximity Label-MS Homo sapiens
24 LZTR1 8216
Affinity Capture-MS Homo sapiens
25 SNRPC 6631
Affinity Capture-MS Homo sapiens
26 PPP3CC 5533
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PARD3B is involved
No pathways found





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