Gene description for PARP3
Gene name poly (ADP-ribose) polymerase family, member 3
Gene symbol PARP3
Other names/aliases ADPRT3
ADPRTL2
ADPRTL3
ARTD3
IRT1
PADPRT-3
Species Homo sapiens
 Database cross references - PARP3
ExoCarta ExoCarta_10039
Vesiclepedia VP_10039
Entrez Gene 10039
HGNC 273
MIM 607726
UniProt Q9Y6F1  
 PARP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PARP3
Molecular Function
    catalytic activity GO:0003824 TAS
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IBA
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 IDA
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    NAD DNA ADP-ribosyltransferase activity GO:0140294 IDA
    NAD+-protein-lysine ADP-ribosyltransferase activity GO:0140804 IDA
    NAD+-protein-aspartate ADP-ribosyltransferase activity GO:0140806 IDA
    NAD+-protein-glutamate ADP-ribosyltransferase activity GO:0140807 IDA
    NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity GO:0140822 IEA
    NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity GO:0140844 IEA
    NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity GO:0140867 IEA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IBA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 IDA
Biological Process
    telomere maintenance GO:0000723 IMP
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 IDA
    double-strand break repair GO:0006302 IMP
    DNA ADP-ribosylation GO:0030592 IDA
    negative regulation of telomere maintenance via telomerase GO:0032211 TAS
    negative regulation of isotype switching GO:0045829 ISS
    positive regulation of DNA ligation GO:0051106 IGI
    regulation of mitotic spindle organization GO:0060236 IMP
    protein poly-ADP-ribosylation GO:0070212 IDA
    protein auto-ADP-ribosylation GO:0070213 IDA
    protein localization to site of double-strand break GO:1990166 IMP
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IBA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 IEA
    centriole GO:0005814 IEA
    nuclear body GO:0016604 IDA
    site of double-strand break GO:0035861 IBA
    site of double-strand break GO:0035861 IDA
    intercellular bridge GO:0045171 IDA
 Experiment description of studies that identified PARP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PARP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 HIST1H1A 3024
Biochemical Activity Homo sapiens
3 PARP3 10039
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 MTHFD2 10797
Affinity Capture-MS Homo sapiens
6 RALGAPB 57148
Affinity Capture-MS Homo sapiens
7 ATM 472
Co-localization Homo sapiens
8 PAK1 5058
Affinity Capture-MS Homo sapiens
9 PARP1 142
Reconstituted Complex Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
10 RPA3 6119
Affinity Capture-MS Homo sapiens
11 APLF  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
12 HIST2H3C 126961
Affinity Capture-MS Homo sapiens
13 DMWD  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PARP3 is involved
No pathways found





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