Gene description for Rpl11
Gene name ribosomal protein L11
Gene symbol Rpl11
Other names/aliases 2010203J19Rik
Species Mus musculus
 Database cross references - Rpl11
ExoCarta ExoCarta_67025
Vesiclepedia VP_67025
Entrez Gene 67025
UniProt Q9CXW4  
 Rpl11 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl11
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    5S rRNA binding GO:0008097 IEA
    5S rRNA binding GO:0008097 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    peroxisome proliferator activated receptor binding GO:0042975 ISO
    ubiquitin ligase inhibitor activity GO:1990948 IEA
    ubiquitin ligase inhibitor activity GO:1990948 ISO
Biological Process
    ribosomal large subunit assembly GO:0000027 IEA
    ribosomal large subunit assembly GO:0000027 ISO
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 IEA
    rRNA processing GO:0006364 ISO
    translation GO:0006412 IBA
    protein targeting GO:0006605 IEA
    protein targeting GO:0006605 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of protein binding GO:0032092 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IMP
    protein localization to nucleus GO:0034504 IMP
    ribosomal large subunit biogenesis GO:0042273 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    regulation of signal transduction by p53 class mediator GO:1901796 ISO
    positive regulation of signal transduction by p53 class mediator GO:1901798 IMP
    negative regulation of ubiquitin protein ligase activity GO:1904667 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
    negative regulation of protein neddylation GO:2000435 IEA
    negative regulation of protein neddylation GO:2000435 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    protein-containing complex GO:0032991 ISO
    synapse GO:0045202 ISO
 Experiment description of studies that identified Rpl11 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Mdm2  
Affinity Capture-Western Mus musculus
4 Rpl35 66489
Co-fractionation Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 Rpl38  
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Rps15a 267019
Co-fractionation Mus musculus
9 Tmem173  
Proximity Label-MS Mus musculus
10 Rpl28 19943
Co-fractionation Mus musculus
11 Rpl13a 22121
Co-fractionation Mus musculus
12 Rpl15 66480
Co-fractionation Mus musculus
13 Rplp2 67186
Co-fractionation Mus musculus
14 Gnb2l1 14694
Co-fractionation Mus musculus
15 Rps4x 20102
Co-fractionation Mus musculus
16 Rpl4 67891
Co-fractionation Mus musculus
17 Rpl14 67115
Co-fractionation Mus musculus
18 Rpl10a 19896
Co-fractionation Mus musculus
19 Rpl32 19951
Co-fractionation Mus musculus
20 Rps8 20116
Co-fractionation Mus musculus
21 Rps23 66475
Co-fractionation Mus musculus
22 Rpl7a 27176
Co-fractionation Mus musculus
23 Rps18 20084
Co-fractionation Mus musculus
24 Rpl27 19942
Co-fractionation Mus musculus
25 Rbm8a  
Co-fractionation Mus musculus
26 Atxn1  
Proximity Label-MS Mus musculus
27 Rpl6 19988
Co-fractionation Mus musculus
28 Kctd13  
Affinity Capture-MS Mus musculus
29 Rps19 20085
Co-fractionation Mus musculus
30 Rpl9 20005
Co-fractionation Mus musculus
31 Rpl3 27367
Co-fractionation Mus musculus
32 Rpl24 68193
Co-fractionation Mus musculus
33 Tpt1 22070
Co-fractionation Mus musculus
34 Rpgrip1l  
Affinity Capture-MS Mus musculus
35 Rps11 27207
Co-fractionation Mus musculus
36 Rpl18a 76808
Co-fractionation Mus musculus
37 Rps24 20088
Co-fractionation Mus musculus
38 Rps10 67097
Co-fractionation Mus musculus
39 Rpl17 319195
Co-fractionation Mus musculus
40 Rpl22 19934
Co-fractionation Mus musculus
41 Rps16 20055
Co-fractionation Mus musculus
42 Ppif  
Co-fractionation Mus musculus
43 Fzr1  
Affinity Capture-MS Mus musculus
44 Upf1 19704
Co-fractionation Mus musculus
45 Eef2 13629
Co-fractionation Mus musculus
46 Rps20 67427
Co-fractionation Mus musculus
47 Rps13 68052
Co-fractionation Mus musculus
48 Rpl12 269261
Co-fractionation Mus musculus
49 Eef1a1 13627
Co-fractionation Mus musculus
50 Rpl21 19933
Co-fractionation Mus musculus
51 Rps21 66481
Co-fractionation Mus musculus
52 Rpl7 19989
Co-fractionation Mus musculus
53 Rpl39 67248
Co-fractionation Mus musculus
54 Rps28 54127
Co-fractionation Mus musculus
55 Rplp0 11837
Co-fractionation Mus musculus
56 Rps15 20054
Co-fractionation Mus musculus
57 Rps5 20103
Co-fractionation Mus musculus
58 Rps7 20115
Co-fractionation Mus musculus
59 Rps3a1 20091
Co-fractionation Mus musculus
60 Rpl36a 19982
Co-fractionation Mus musculus
61 Rpl18 19899
Co-fractionation Mus musculus
62 Rpl26 19941
Co-fractionation Mus musculus
63 Fancd2  
Affinity Capture-MS Mus musculus
64 Rpl23 65019
Co-fractionation Mus musculus
65 Rpl23a 268449
Co-fractionation Mus musculus
66 Rps14 20044
Co-fractionation Mus musculus
67 Rps17 20068
Co-fractionation Mus musculus
68 Rps9 76846
Co-fractionation Mus musculus
69 Rpl30 19946
Co-fractionation Mus musculus
70 Eed  
Affinity Capture-MS Mus musculus
71 Kcna3  
Affinity Capture-MS Mus musculus
72 Rpsa 16785
Co-fractionation Mus musculus
73 Eef1b2 55949
Co-fractionation Mus musculus
74 Mapt  
Affinity Capture-MS Mus musculus
75 Igsf8 140559
Co-fractionation Mus musculus
76 Rpl5 100503670
Co-fractionation Mus musculus
77 Rpl13 270106
Co-fractionation Mus musculus
78 Rpl8 26961
Co-fractionation Mus musculus
79 Lipc  
Affinity Capture-MS Mus musculus
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