Gene description for Rps18
Gene name ribosomal protein S18
Gene symbol Rps18
Other names/aliases H-2Ke3
H2-Ke3
Ke-3
ke3
Species Mus musculus
 Database cross references - Rps18
ExoCarta ExoCarta_20084
Vesiclepedia VP_20084
Entrez Gene 20084
UniProt P62270  
 Rps18 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps18
Molecular Function
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    rRNA binding GO:0019843 IEA
    protein kinase binding GO:0019901 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    small ribosomal subunit GO:0015935 IBA
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rps18 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Nphs2  
Affinity Capture-MS Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 Eif3a 13669
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Rps10 67097
Co-fractionation Mus musculus
10 Rpl13a 22121
Co-fractionation Mus musculus
11 Rps4x 20102
Co-fractionation Mus musculus
12 Rpl15 66480
Co-fractionation Mus musculus
13 Rplp2 67186
Co-fractionation Mus musculus
14 Rpl38  
Co-fractionation Mus musculus
15 Rps19 20085
Co-fractionation Mus musculus
16 Rpl4 67891
Co-fractionation Mus musculus
17 Rpl14 67115
Co-fractionation Mus musculus
18 Rps2 16898
Co-fractionation Mus musculus
19 Rpl10a 19896
Co-fractionation Mus musculus
20 Rps8 20116
Co-fractionation Mus musculus
21 Rps23 66475
Co-fractionation Mus musculus
22 Rpl7a 27176
Co-fractionation Mus musculus
23 Rpl32 19951
Co-fractionation Mus musculus
24 Wibg  
Co-fractionation Mus musculus
25 Rpl6 19988
Co-fractionation Mus musculus
26 Kctd13  
Affinity Capture-MS Mus musculus
27 Rpl19 19921
Co-fractionation Mus musculus
28 Rps26 27370
Co-fractionation Mus musculus
29 Rpl35 66489
Co-fractionation Mus musculus
30 Tpt1 22070
Co-fractionation Mus musculus
31 Rps11 27207
Co-fractionation Mus musculus
32 Rpl18a 76808
Co-fractionation Mus musculus
33 Pabpc1 18458
Co-fractionation Mus musculus
34 Rps5 20103
Co-fractionation Mus musculus
35 Rpl13 270106
Co-fractionation Mus musculus
36 Rps7 20115
Co-fractionation Mus musculus
37 Rpl17 319195
Co-fractionation Mus musculus
38 Rpl22 19934
Co-fractionation Mus musculus
39 Rpl5 100503670
Co-fractionation Mus musculus
40 Fzr1  
Affinity Capture-MS Mus musculus
41 Eef2 13629
Co-fractionation Mus musculus
42 Rps24 20088
Co-fractionation Mus musculus
43 Rps13 68052
Co-fractionation Mus musculus
44 Rpl7 19989
Co-fractionation Mus musculus
45 Eef1a1 13627
Co-fractionation Mus musculus
46 Rps21 66481
Co-fractionation Mus musculus
47 Rpl12 269261
Co-fractionation Mus musculus
48 Rps15a 267019
Co-fractionation Mus musculus
49 Gnb2l1 14694
Co-fractionation Mus musculus
50 Rps15 20054
Co-fractionation Mus musculus
51 Rpl18 19899
Co-fractionation Mus musculus
52 Rps3a1 20091
Co-fractionation Mus musculus
53 Rpl26 19941
Co-fractionation Mus musculus
54 Hba 15121
Co-fractionation Mus musculus
55 Rplp0 11837
Co-fractionation Mus musculus
56 Rpl23a 268449
Co-fractionation Mus musculus
57 Rps14 20044
Co-fractionation Mus musculus
58 Rps6 20104
Co-fractionation Mus musculus
59 Rpl30 19946
Co-fractionation Mus musculus
60 Eed  
Affinity Capture-MS Mus musculus
61 Rps27 57294
Co-fractionation Mus musculus
62 Rpsa 16785
Co-fractionation Mus musculus
63 Eef1b2 55949
Co-fractionation Mus musculus
64 Rpl3 27367
Co-fractionation Mus musculus
65 Rps16 20055
Co-fractionation Mus musculus
66 Rps20 67427
Co-fractionation Mus musculus
67 Rps28 54127
Co-fractionation Mus musculus
68 Rps12 20042
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here