Gene description for Rpl24
Gene name ribosomal protein L24
Gene symbol Rpl24
Other names/aliases 0610008L05Rik
Bst
Species Mus musculus
 Database cross references - Rpl24
ExoCarta ExoCarta_68193
Vesiclepedia VP_68193
Entrez Gene 68193
UniProt Q8BP67  
 Rpl24 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl24
Molecular Function
    mRNA binding GO:0003729 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    structural constituent of ribosome GO:0003735 ISS
Biological Process
    ribosomal large subunit assembly GO:0000027 IMP
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 IMP
    translation GO:0006412 ISO
    mitotic cell cycle checkpoint signaling GO:0007093 IMP
    exit from mitosis GO:0010458 IMP
    optic nerve development GO:0021554 IMP
    retinal ganglion cell axon guidance GO:0031290 IMP
    retina development in camera-type eye GO:0060041 IMP
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl24 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Rpl35 66489
Co-fractionation Mus musculus
4 Cbx1  
Affinity Capture-MS Mus musculus
5 Rplp0 11837
Co-fractionation Mus musculus
6 Rpl31 114641
Co-fractionation Mus musculus
7 Rps15a 267019
Co-fractionation Mus musculus
8 Rps26 27370
Co-fractionation Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Rpl15 66480
Co-fractionation Mus musculus
11 Rplp2 67186
Co-fractionation Mus musculus
12 Rpl38  
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Rpl4 67891
Co-fractionation Mus musculus
15 Rpl14 67115
Co-fractionation Mus musculus
16 Rps2 16898
Co-fractionation Mus musculus
17 Rps15 20054
Co-fractionation Mus musculus
18 Rpl10a 19896
Co-fractionation Mus musculus
19 Rps23 66475
Co-fractionation Mus musculus
20 Rpl7a 27176
Co-fractionation Mus musculus
21 Rpl27 19942
Co-fractionation Mus musculus
22 Rpl32 19951
Co-fractionation Mus musculus
23 Faf1  
Co-fractionation Mus musculus
24 Rpl6 19988
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Eef1g 67160
Co-fractionation Mus musculus
27 Tpt1 22070
Co-fractionation Mus musculus
28 Rps11 27207
Co-fractionation Mus musculus
29 Rpl18a 76808
Co-fractionation Mus musculus
30 Rpl11 67025
Co-fractionation Mus musculus
31 Rpl13 270106
Co-fractionation Mus musculus
32 Rpl17 319195
Co-fractionation Mus musculus
33 Rpl22 19934
Co-fractionation Mus musculus
34 Mettl21e  
Affinity Capture-MS Mus musculus
35 Rps16 20055
Co-fractionation Mus musculus
36 Tmem173  
Proximity Label-MS Mus musculus
37 Fzr1  
Affinity Capture-MS Mus musculus
38 EIF4ENIF1 56478
Two-hybrid Homo sapiens
39 Rps24 20088
Co-fractionation Mus musculus
40 Ranbp3  
Co-fractionation Mus musculus
41 Rpl7 19989
Co-fractionation Mus musculus
42 Rpl18 19899
Co-fractionation Mus musculus
43 Rpl21 19933
Co-fractionation Mus musculus
44 Pcbp1 23983
Two-hybrid Mus musculus
45 Rps21 66481
Co-fractionation Mus musculus
46 Rpl39 67248
Co-fractionation Mus musculus
47 Rps28 54127
Co-fractionation Mus musculus
48 Gnb2l1 14694
Co-fractionation Mus musculus
49 Syncrip 56403
Co-fractionation Mus musculus
50 Rps5 20103
Co-fractionation Mus musculus
51 Rps3a1 20091
Co-fractionation Mus musculus
52 Cbx3 12417
Affinity Capture-MS Mus musculus
53 Rpl36a 19982
Co-fractionation Mus musculus
54 Rps9 76846
Co-fractionation Mus musculus
55 Rpl26 19941
Co-fractionation Mus musculus
56 Nars 70223
Co-fractionation Mus musculus
57 Rpl23 65019
Co-fractionation Mus musculus
58 Rpl23a 268449
Co-fractionation Mus musculus
59 Rps14 20044
Co-fractionation Mus musculus
60 Rps17 20068
Co-fractionation Mus musculus
61 Rps6 20104
Co-fractionation Mus musculus
62 Rpl30 19946
Co-fractionation Mus musculus
63 Eed  
Affinity Capture-MS Mus musculus
64 Rpsa 16785
Co-fractionation Mus musculus
65 Eef1b2 55949
Co-fractionation Mus musculus
66 Rpl3 27367
Co-fractionation Mus musculus
67 Rpl5 100503670
Co-fractionation Mus musculus
68 Fancd2  
Affinity Capture-MS Mus musculus
69 Dynlt1b  
Two-hybrid Mus musculus
70 Rpl8 26961
Co-fractionation Mus musculus
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