Experiment description of studies that identified Tpt1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Tpt1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Rps27a 78294 Co-fractionation Mus musculus 2 Rps3 27050 Co-fractionation Mus musculus 3 Rpl11 67025 Co-fractionation Mus musculus 4 Rpl35 66489 Co-fractionation Mus musculus 5 Rpl31 114641 Co-fractionation Mus musculus 6 Rps25 75617 Co-fractionation Mus musculus 7 Rps28 54127 Co-fractionation Mus musculus 8 Rps10 67097 Co-fractionation Mus musculus 9 Rpl13a 22121 Co-fractionation Mus musculus 10 Snx6 72183 Co-fractionation Mus musculus 11 Eif5a 276770 Co-fractionation Mus musculus 12 Rplp2 67186 Co-fractionation Mus musculus 13 Rpl38   Co-fractionation Mus musculus 14 Rps4x 20102 Co-fractionation Mus musculus 15 Rpl4 67891 Co-fractionation Mus musculus 16 Rpl14 67115 Co-fractionation Mus musculus 17 Rps2 16898 Co-fractionation Mus musculus 18 Rpl10a 19896 Co-fractionation Mus musculus 19 Kcnma1   Affinity Capture-MS Mus musculus 20 Rps8 20116 Co-fractionation Mus musculus 21 Pkm 18746 Co-fractionation Mus musculus 22 Rps23 66475 Co-fractionation Mus musculus 23 Rpl7a 27176 Co-fractionation Mus musculus 24 Rps18 20084 Co-fractionation Mus musculus 25 Rpl27 19942 Co-fractionation Mus musculus 26 Rpl32 19951 Co-fractionation Mus musculus 27 Rpl6 19988 Co-fractionation Mus musculus 28 Rpl19 19921 Co-fractionation Mus musculus 29 Tfe3   Affinity Capture-MS Mus musculus 30 Rpl24 68193 Co-fractionation Mus musculus 31 Rps11 27207 Co-fractionation Mus musculus 32 Rpl18a 76808 Co-fractionation Mus musculus 33 Rps24 20088 Co-fractionation Mus musculus 34 Actb 11461 Co-fractionation Mus musculus 35 LSM5   Two-hybrid Homo sapiens 36 Pabpc1 18458 Co-fractionation Mus musculus 37 Rps5 20103 Co-fractionation Mus musculus 38 Rps7 20115 Co-fractionation Mus musculus 39 Rpl17 319195 Co-fractionation Mus musculus 40 Rpl5 100503670 Co-fractionation Mus musculus 41 Rps17 20068 Co-fractionation Mus musculus 42 Eef2 13629 Co-fractionation Mus musculus 43 Rps6 20104 Co-fractionation Mus musculus 44 Src 20779 Co-fractionation Mus musculus 45 Rpl7 19989 Co-fractionation Mus musculus 46 Eef1a1 13627 Co-fractionation Mus musculus 47 Ncl 17975 Affinity Capture-Western Mus musculus Affinity Capture-Luminescence Mus musculus 48 Rheb   Co-fractionation Mus musculus 49 Rps21 66481 Co-fractionation Mus musculus 50 Gnb2l1 14694 Co-fractionation Mus musculus 51 Rps15 20054 Co-fractionation Mus musculus 52 Pgk2 18663 Co-fractionation Mus musculus 53 Rps3a1 20091 Co-fractionation Mus musculus 54 Rpl26 19941 Co-fractionation Mus musculus 55 Rpl13 270106 Co-fractionation Mus musculus 56 Rplp0 11837 Co-fractionation Mus musculus 57 Rpl23a 268449 Co-fractionation Mus musculus 58 Rps14 20044 Co-fractionation Mus musculus 59 Pgk1 18655 Co-fractionation Mus musculus 60 Rpl30 19946 Co-fractionation Mus musculus 61 Eed   Affinity Capture-MS Mus musculus 62 Rpsa 16785 Co-fractionation Mus musculus 63 Eef1b2 55949 Co-fractionation Mus musculus 64 Rps16 20055 Co-fractionation Mus musculus 65 Rps20 67427 Co-fractionation Mus musculus 66 Rpl8 26961 Co-fractionation Mus musculus View the network

Pathways in which Tpt1 is involved

No pathways found