Gene description for UBXN7
Gene name UBX domain protein 7
Gene symbol UBXN7
Other names/aliases UBXD7
Species Homo sapiens
 Database cross references - UBXN7
ExoCarta ExoCarta_26043
Vesiclepedia VP_26043
Entrez Gene 26043
HGNC 29119
UniProt O94888  
 UBXN7 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for UBXN7
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IDA
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IDA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 IDA
 Experiment description of studies that identified UBXN7 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for UBXN7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Co-fractionation Homo sapiens
2 C9orf64 84267
Affinity Capture-MS Homo sapiens
3 OXCT1 5019
Co-fractionation Homo sapiens
4 KLHDC10 23008
Affinity Capture-MS Homo sapiens
5 NPLOC4 55666
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
6 TCEB1 6921
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 VAPA 9218
Affinity Capture-MS Homo sapiens
8 ATG9A 79065
Affinity Capture-MS Homo sapiens
9 KCTD10 83892
Affinity Capture-MS Homo sapiens
10 ZPR1 8882
Co-fractionation Homo sapiens
11 REL 5966
Two-hybrid Homo sapiens
12 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
13 ABCF1 23
Affinity Capture-MS Homo sapiens
14 BIRC2  
Affinity Capture-MS Homo sapiens
15 MORF4L2  
Affinity Capture-MS Homo sapiens
16 USP9X 8239
Co-fractionation Homo sapiens
17 UBC 7316
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 VHL  
Affinity Capture-MS Homo sapiens
19 TRIM62  
Two-hybrid Homo sapiens
20 WDR26 80232
Affinity Capture-MS Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 APP 351
Reconstituted Complex Homo sapiens
23 BRWD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CENPA  
Proximity Label-MS Homo sapiens
25 KLHDC2  
Affinity Capture-MS Homo sapiens
26 VPS26A 9559
Co-fractionation Homo sapiens
27 UFM1 51569
Co-fractionation Homo sapiens
28 KPNA3 3839
Affinity Capture-MS Homo sapiens
29 BRWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 UCHL3 7347
Co-fractionation Homo sapiens
31 SYT2  
Affinity Capture-MS Homo sapiens
32 UBQLN1 29979
Two-hybrid Homo sapiens
33 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TULP3 7289
Affinity Capture-MS Homo sapiens
35 FAF1 11124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
36 UFC1 51506
Co-fractionation Homo sapiens
37 SEC23A 10484
Co-fractionation Homo sapiens
38 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 KCTD13 253980
Affinity Capture-MS Homo sapiens
41 TRIM8  
Two-hybrid Homo sapiens
42 UBE2V1 7335
Co-fractionation Homo sapiens
43 ZYX 7791
Co-fractionation Homo sapiens
44 UBR4 23352
Affinity Capture-MS Homo sapiens
45 KIFC1 3833
Affinity Capture-MS Homo sapiens
46 NSUN2 54888
Co-fractionation Homo sapiens
47 RAD23B 5887
Co-fractionation Homo sapiens
48 DPP8 54878
Co-fractionation Homo sapiens
49 UBE4B 10277
Co-fractionation Homo sapiens
50 CUL4A 8451
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 DAZAP2  
Two-hybrid Homo sapiens
52 RBX1 9978
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 AMFR 267
Affinity Capture-MS Homo sapiens
55 GOLGA8G  
Affinity Capture-MS Homo sapiens
56 CBX3 11335
Proximity Label-MS Homo sapiens
57 UBR5 51366
Affinity Capture-MS Homo sapiens
58 KCNK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PSMD2 5708
Affinity Capture-Western Homo sapiens
60 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
61 CYSRT1 375791
Two-hybrid Homo sapiens
62 NTRK1 4914
Affinity Capture-MS Homo sapiens
63 VPRBP 9730
Affinity Capture-MS Homo sapiens
64 ACTB 60
Affinity Capture-MS Homo sapiens
65 CSNK1E 1454
Affinity Capture-MS Homo sapiens
66 XKRX  
Affinity Capture-MS Homo sapiens
67 TGIF2LY  
Affinity Capture-MS Homo sapiens
68 CUL2 8453
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
69 VCPIP1 80124
Affinity Capture-MS Homo sapiens
70 YAP1 10413
Co-fractionation Homo sapiens
71 SETD7 80854
Affinity Capture-MS Homo sapiens
72 TTLL12 23170
Co-fractionation Homo sapiens
73 UBR2 23304
Affinity Capture-MS Homo sapiens
74 POU3F2  
Affinity Capture-MS Homo sapiens
75 CSNK1G3 1456
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 FAM189B  
Affinity Capture-MS Homo sapiens
77 NUAK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 UFD1L 7353
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
79 WDR11 55717
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 TRAF6 7189
Two-hybrid Homo sapiens
81 CSTF1 1477
Affinity Capture-MS Homo sapiens
82 VAPB 9217
Affinity Capture-MS Homo sapiens
83 CUL3 8452
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 NUBP2 10101
Co-fractionation Homo sapiens
85 TYMS 7298
Co-fractionation Homo sapiens
86 SRXN1 140809
Co-fractionation Homo sapiens
87 UBE2L3 7332
Co-fractionation Homo sapiens
88 ACAD11 84129
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
89 PHIP 55023
Affinity Capture-MS Homo sapiens
90 ERCC8  
Affinity Capture-Western Homo sapiens
91 ALPP 250
Affinity Capture-MS Homo sapiens
92 MAP3K1 4214
Biochemical Activity Homo sapiens
93 NSFL1C 55968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 KBTBD7  
Affinity Capture-MS Homo sapiens
95 ZFYVE19 84936
Co-fractionation Homo sapiens
96 KLHL13 90293
Affinity Capture-MS Homo sapiens
97 CRX  
Two-hybrid Homo sapiens
98 RNF138  
Affinity Capture-MS Homo sapiens
99 JUN 3725
Affinity Capture-MS Homo sapiens
100 PAX5  
Two-hybrid Homo sapiens
101 UBE2D2 7322
Co-fractionation Homo sapiens
102 UBR1 197131
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
103 FAM91A1 157769
Affinity Capture-MS Homo sapiens
104 LMNB1 4001
Proximity Label-MS Homo sapiens
105 PHF6  
Affinity Capture-MS Homo sapiens
106 TCEB2 6923
Affinity Capture-MS Homo sapiens
107 DDB1 1642
Affinity Capture-MS Homo sapiens
108 TRIM39  
Two-hybrid Homo sapiens
109 PLEKHG3 26030
Affinity Capture-MS Homo sapiens
110 LMNA 4000
Proximity Label-MS Homo sapiens
111 UBXN1 51035
Affinity Capture-MS Homo sapiens
112 UBE2D1 7321
Co-fractionation Homo sapiens
113 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
114 SDHAF2 54949
Co-fractionation Homo sapiens
115 UBQLN2 29978
Two-hybrid Homo sapiens
116 PARP1 142
Proximity Label-MS Homo sapiens
117 ZRANB2 9406
Co-fractionation Homo sapiens
118 USP34 9736
Co-fractionation Homo sapiens
119 TNFAIP1 7126
Affinity Capture-MS Homo sapiens
120 SULT1A1 6817
Co-fractionation Homo sapiens
121 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 MEN1 4221
Affinity Capture-MS Homo sapiens
123 SKP1 6500
Affinity Capture-MS Homo sapiens
124 SMURF1 57154
Biochemical Activity Homo sapiens
125 ZNRF1  
Two-hybrid Homo sapiens
126 UBE2C 11065
Co-fractionation Homo sapiens
127 RANBP6 26953
Affinity Capture-MS Homo sapiens
128 PJA2  
Affinity Capture-MS Homo sapiens
129 LZTFL1 54585
Co-fractionation Homo sapiens
130 FMNL3 91010
Affinity Capture-MS Homo sapiens
131 WIF1 11197
Affinity Capture-MS Homo sapiens
132 ATP6V1F 9296
Co-fractionation Homo sapiens
133 APPBP2  
Affinity Capture-MS Homo sapiens
134 ERCC3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
135 NUP50 10762
Proximity Label-MS Homo sapiens
136 CLEC19A  
Two-hybrid Homo sapiens
137 UBE2B 7320
Co-fractionation Homo sapiens
138 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 SSSCA1 10534
Co-fractionation Homo sapiens
140 RNF111 54778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 KLHL22 84861
Affinity Capture-MS Homo sapiens
142 TOPORS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 KBTBD2 25948
Affinity Capture-MS Homo sapiens
144 TMEM31  
Affinity Capture-MS Homo sapiens
145 BTBD9  
Affinity Capture-MS Homo sapiens
146 KLHL12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 CAV1 857
Affinity Capture-MS Homo sapiens
148 UBE2V2 7336
Co-fractionation Homo sapiens
149 NEDD8 4738
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
150 NHLH1  
Proximity Label-MS Homo sapiens
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