Gene description for ESAM
Gene name endothelial cell adhesion molecule
Gene symbol ESAM
Other names/aliases W117m
Species Homo sapiens
 Database cross references - ESAM
ExoCarta ExoCarta_90952
Vesiclepedia VP_90952
Entrez Gene 90952
HGNC 17474
MIM 614281
UniProt Q96AP7  
 ESAM identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ESAM
Molecular Function
    protein binding GO:0005515 IPI
    cell-cell adhesion mediator activity GO:0098632 IBA
    cell-cell adhesion mediator activity GO:0098632 IDA
Biological Process
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    protein localization GO:0008104 IDA
    regulation of actin filament polymerization GO:0030833 IDA
    regulation of actin cytoskeleton organization GO:0032956 IDA
    maintenance of blood-brain barrier GO:0035633 NAS
    bicellular tight junction assembly GO:0070830 IMP
    cell-cell adhesion GO:0098609 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IBA
    bicellular tight junction GO:0005923 IEA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified ESAM in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ESAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MDC1  
Affinity Capture-MS Homo sapiens
2 TMBIM6 7009
Two-hybrid Homo sapiens
3 CENPF 1063
Affinity Capture-MS Homo sapiens
4 C3orf52  
Two-hybrid Homo sapiens
5 KIF14 9928
Affinity Capture-MS Homo sapiens
6 MPHOSPH8 54737
Affinity Capture-MS Homo sapiens
7 TOP1 7150
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ESAM is involved
PathwayEvidenceSource
Cell surface interactions at the vascular wall TAS Reactome
Hemostasis TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here