Experiment description of studies that identified PTTG1IP in exosomes

1 Experiment ID 282 ISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81|EpCAM EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 283 ISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81|EpCAM EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

3 Experiment ID 285 ISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81|EpCAM EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 286 ISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81|EpCAM EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

5 Experiment ID 257 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 126 ISEV standards ✘ EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

7 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 231 ISEV standards ✘ EV Biophysical techniques ✔ Alix EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 232 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

11 Experiment ID 233 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

12 Experiment ID 274 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|RAB5A EV Cytosolic markers ✔ CD9|CD82|CD63|CD81 EV Membrane markers ✔ AIF EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel resistant Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Flow cytometry Western blotting

13 Experiment ID 63 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ AQP2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for PTTG1IP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PTTG1   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens View the network

Pathways in which PTTG1IP is involved

No pathways found