Gene description for TESK1
Gene name testis-specific kinase 1
Gene symbol TESK1
Other names/aliases -
Species Homo sapiens
 Database cross references - TESK1
ExoCarta ExoCarta_7016
Vesiclepedia VP_7016
Entrez Gene 7016
HGNC 11731
MIM 601782
UniProt Q15569  
 TESK1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for TESK1
Molecular Function
    protein kinase activity GO:0004672 IMP
    protein kinase activity GO:0004672 ISS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IMP
    chromatin remodeling GO:0006338 IEA
    spermatogenesis GO:0007283 IEA
    actin cytoskeleton organization GO:0030036 IBA
    negative regulation of protein autophosphorylation GO:0031953 ISS
    regulation of protein localization GO:0032880 ISS
    regulation of actin cytoskeleton organization GO:0032956 ISS
    positive regulation of stress fiber assembly GO:0051496 IBA
    positive regulation of stress fiber assembly GO:0051496 ISS
    positive regulation of stress fiber assembly GO:0051496 TAS
    establishment of vesicle localization GO:0051650 IMP
    nuclear-transcribed mRNA catabolic process, no-go decay GO:0070966 ISS
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISS
    podocyte cell migration GO:0090521 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of protein localization to nucleus GO:1900182 IMP
    negative regulation of cilium assembly GO:1902018 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    lamellipodium GO:0030027 IEA
    cytoplasmic vesicle GO:0031410 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
 Experiment description of studies that identified TESK1 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for TESK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PFKL 5211
Two-hybrid Homo sapiens
2 ATG9A 79065
Proximity Label-MS Homo sapiens
3 SPRY3  
Affinity Capture-Western Homo sapiens
4 ABCB4 5244
Two-hybrid Homo sapiens
5 RABEPK  
Affinity Capture-MS Homo sapiens
6 ARAF 369
Two-hybrid Homo sapiens
7 SPRED1 161742
Affinity Capture-Western Homo sapiens
8 SPRY2 10253
Affinity Capture-Western Homo sapiens
9 SPRY4 81848
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 YY1 7528
Two-hybrid Homo sapiens
11 SPRED2 200734
Affinity Capture-Western Homo sapiens
12 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
13 SPRY1 10252
Affinity Capture-Western Homo sapiens
14 YWHAB 7529
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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