Gene description for PON1
Gene name paraoxonase 1
Gene symbol PON1
Other names/aliases ESA
MVCD5
PON
Species Homo sapiens
 Database cross references - PON1
ExoCarta ExoCarta_5444
Vesiclepedia VP_5444
Entrez Gene 5444
HGNC 9204
MIM 168820
UniProt P27169  
 PON1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PON1
Molecular Function
    aryldialkylphosphatase activity GO:0004063 IBA
    aryldialkylphosphatase activity GO:0004063 IDA
    arylesterase activity GO:0004064 IBA
    arylesterase activity GO:0004064 IDA
    arylesterase activity GO:0004064 NAS
    calcium ion binding GO:0005509 IDA
    phospholipid binding GO:0005543 IDA
    protein homodimerization activity GO:0042803 IPI
    acyl-L-homoserine-lactone lactonohydrolase activity GO:0102007 IDA
Biological Process
    cholesterol metabolic process GO:0008203 IEA
    response to toxic substance GO:0009636 IBA
    response to toxic substance GO:0009636 IDA
    positive regulation of cholesterol efflux GO:0010875 IDA
    negative regulation of plasma lipoprotein oxidation GO:0034445 IDA
    carboxylic acid catabolic process GO:0046395 IDA
    organophosphate catabolic process GO:0046434 IDA
    phosphatidylcholine metabolic process GO:0046470 IDA
    lactone catabolic process GO:1901335 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    high-density lipoprotein particle GO:0034364 IDA
    high-density lipoprotein particle GO:0034364 TAS
    spherical high-density lipoprotein particle GO:0034366 IDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified PON1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PON1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Biochemical Activity Homo sapiens
2 RDH13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MICU2 221154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NFYC 4802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PON3 5446
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MATN2 4147
Affinity Capture-MS Homo sapiens
7 NDUFB6 4712
Affinity Capture-MS Homo sapiens
8 KLK10 5655
Affinity Capture-MS Homo sapiens
9 FANCD2  
Affinity Capture-MS Homo sapiens
10 TRIM21 6737
Affinity Capture-MS Homo sapiens
11 SFXN5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CLU 1191
Affinity Capture-Western Homo sapiens
13 UBE2U  
Affinity Capture-MS Homo sapiens
14 PLK1 5347
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
15 PHF11 51131
Affinity Capture-MS Homo sapiens
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