Gene description for DSE
Gene name dermatan sulfate epimerase
Gene symbol DSE
Other names/aliases DSEP
DSEPI
EDSMC2
SART-2
SART2
Species Homo sapiens
 Database cross references - DSE
ExoCarta ExoCarta_29940
Vesiclepedia VP_29940
Entrez Gene 29940
HGNC 21144
MIM 605942
UniProt Q9UL01  
 DSE identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DSE
Molecular Function
    metal ion binding GO:0046872 IEA
    chondroitin-glucuronate 5-epimerase activity GO:0047757 IBA
    chondroitin-glucuronate 5-epimerase activity GO:0047757 IDA
    chondroitin-glucuronate 5-epimerase activity GO:0047757 TAS
Biological Process
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IEA
    chondroitin sulfate metabolic process GO:0030204 IBA
    dermatan sulfate metabolic process GO:0030205 IBA
    chondroitin sulfate biosynthetic process GO:0030206 IEA
    dermatan sulfate biosynthetic process GO:0030208 IDA
    dermatan sulfate biosynthetic process GO:0030208 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
 Experiment description of studies that identified DSE in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DSE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MCOLN2  
Affinity Capture-MS Homo sapiens
4 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GNPTAB 79158
Affinity Capture-MS Homo sapiens
6 TMEM59 9528
Affinity Capture-MS Homo sapiens
7 SIDT2 51092
Affinity Capture-MS Homo sapiens
8 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
10 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TMEM186  
Affinity Capture-MS Homo sapiens
12 SLC7A14  
Affinity Capture-MS Homo sapiens
13 FAXC  
Affinity Capture-MS Homo sapiens
14 NRBP1 29959
Affinity Capture-MS Homo sapiens
15 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CLEC12B  
Affinity Capture-MS Homo sapiens
17 CHRNB2  
Affinity Capture-MS Homo sapiens
18 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
19 PARS2 25973
Affinity Capture-MS Homo sapiens
20 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CD1E  
Affinity Capture-MS Homo sapiens
22 SCGB2A2  
Affinity Capture-MS Homo sapiens
23 C1orf54  
Affinity Capture-MS Homo sapiens
24 CPSF3L 54973
Two-hybrid Homo sapiens
25 ACSL4 2182
Two-hybrid Homo sapiens
26 CHRNA3 1136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here