Gene description for GTF3A
Gene name general transcription factor IIIA
Gene symbol GTF3A
Other names/aliases AP2
TFIIIA
Species Homo sapiens
 Database cross references - GTF3A
ExoCarta ExoCarta_2971
Vesiclepedia VP_2971
Entrez Gene 2971
HGNC 4662
MIM 600860
UniProt Q92664  
 GTF3A identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GTF3A
Molecular Function
    DNA binding GO:0003677 IEA
    5S rRNA binding GO:0008097 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    transcription by RNA polymerase III GO:0006383 TAS
    rRNA transcription GO:0009303 TAS
    ribosomal large subunit biogenesis GO:0042273 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified GTF3A in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GTF3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSB 6741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RALBP1 10928
Affinity Capture-Western Homo sapiens
3 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
4 GTF3C3 9330
Affinity Capture-MS Homo sapiens
5 EGFR 1956
Negative Genetic Homo sapiens
6 GTF3C5 9328
Affinity Capture-MS Homo sapiens
7 CXCR2  
Affinity Capture-Western Homo sapiens
8 EPN1 29924
Affinity Capture-Western Homo sapiens
9 CHMP5 51510
Two-hybrid Homo sapiens
10 PRLR  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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