Gene description for H2afy
Gene name H2A histone family, member Y
Gene symbol H2afy
Other names/aliases H2AF12M
mH2a1
macroH2A1
Species Mus musculus
 Database cross references - H2afy
ExoCarta ExoCarta_26914
Vesiclepedia VP_26914
Entrez Gene 26914
UniProt Q9QZQ8  
 H2afy identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for H2afy
Molecular Function
    rDNA binding GO:0000182 IEA
    rDNA binding GO:0000182 ISO
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IEA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    chromatin binding GO:0003682 IDA
    double-stranded methylated DNA binding GO:0010385 IEA
    double-stranded methylated DNA binding GO:0010385 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein serine/threonine kinase inhibitor activity GO:0030291 IEA
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    structural constituent of chromatin GO:0030527 IBA
    structural constituent of chromatin GO:0030527 IEA
    structural constituent of chromatin GO:0030527 ISO
    chromatin DNA binding GO:0031490 ISO
    nucleosomal DNA binding GO:0031492 IDA
    nucleosomal DNA binding GO:0031492 ISO
    protein heterodimerization activity GO:0046982 IEA
    ADP-D-ribose binding GO:0072570 IDA
    ADP-D-ribose binding GO:0072570 ISO
    ADP-D-ribose modification-dependent protein binding GO:0160002 IDA
    promoter-specific chromatin binding GO:1990841 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    regulation of oxidative phosphorylation GO:0002082 IDA
    DNA repair GO:0006281 IEA
    DNA repair GO:0006281 ISO
    nucleosome assembly GO:0006334 IEA
    sex-chromosome dosage compensation GO:0007549 ISO
    regulation of lipid metabolic process GO:0019216 IEA
    regulation of lipid metabolic process GO:0019216 ISO
    heterochromatin formation GO:0031507 IBA
    positive regulation of maintenance of mitotic sister chromatid cohesion GO:0034184 IEA
    positive regulation of maintenance of mitotic sister chromatid cohesion GO:0034184 ISO
    epigenetic regulation of gene expression GO:0040029 ISO
    positive regulation of keratinocyte differentiation GO:0045618 IEA
    positive regulation of keratinocyte differentiation GO:0045618 ISO
    negative regulation of gene expression, epigenetic GO:0045814 IEA
    negative regulation of gene expression, epigenetic GO:0045814 ISO
    transcription initiation-coupled chromatin remodeling GO:0045815 IEA
    transcription initiation-coupled chromatin remodeling GO:0045815 ISO
    establishment of protein localization to chromatin GO:0071169 IEA
    establishment of protein localization to chromatin GO:0071169 ISO
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISO
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 IBA
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 IMP
    negative regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901837 ISO
    regulation of NAD metabolic process GO:1902688 IDA
    negative regulation of cell cycle G2/M phase transition GO:1902750 IEA
    negative regulation of cell cycle G2/M phase transition GO:1902750 ISO
    regulation of response to oxidative stress GO:1902882 IMP
    regulation of response to oxidative stress GO:1902882 ISO
    negative regulation of response to oxidative stress GO:1902883 IMP
    negative regulation of response to oxidative stress GO:1902883 ISO
    positive regulation of response to oxidative stress GO:1902884 IMP
    positive regulation of response to oxidative stress GO:1902884 ISO
    positive regulation of endodermal cell differentiation GO:1903226 IEA
    positive regulation of endodermal cell differentiation GO:1903226 ISO
    negative regulation of protein localization to chromosome, telomeric region GO:1904815 IEA
    negative regulation of protein localization to chromosome, telomeric region GO:1904815 ISO
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    chromatin GO:0000785 ISO
    nucleosome GO:0000786 IBA
    nucleosome GO:0000786 IEA
    nucleosome GO:0000786 ISO
    condensed chromosome GO:0000793 IDA
    Barr body GO:0001740 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    pericentric heterochromatin GO:0005721 IEA
    pericentric heterochromatin GO:0005721 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    centrosome GO:0005813 TAS
    site of DNA damage GO:0090734 IEA
    site of DNA damage GO:0090734 ISO
 Experiment description of studies that identified H2afy in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for H2afy
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Phf14  
Co-fractionation Mus musculus
2 Fmr1  
Affinity Capture-MS Mus musculus
3 Ace 11421
Co-fractionation Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Dek 110052
Co-fractionation Mus musculus
6 Unk  
Affinity Capture-RNA Mus musculus
7 Ddb1 13194
Affinity Capture-MS Mus musculus
8 Spop  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which H2afy is involved
No pathways found





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