Gene description for DCAF12
Gene name DDB1 and CUL4 associated factor 12
Gene symbol DCAF12
Other names/aliases CT102
KIAA1892
TCC52
WDR40A
Species Homo sapiens
 Database cross references - DCAF12
ExoCarta ExoCarta_25853
Entrez Gene 25853
HGNC 19911
UniProt Q5T6F0  
 DCAF12 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DCAF12
Biological Process
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    centrosome GO:0005813 IDA
    cytoplasm GO:0005737 IDA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
 Experiment description of studies that identified DCAF12 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DCAF12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DCAF12 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here