Gene description for FBN1
Gene name fibrillin 1
Gene symbol FBN1
Other names/aliases ACMICD
ECTOL1
FBN
GPHYSD2
MASS
MFS1
OCTD
SGS
SSKS
WMS
WMS2
Species Homo sapiens
 Database cross references - FBN1
ExoCarta ExoCarta_2200
Entrez Gene 2200
HGNC 3603
MIM 134797
UniProt P35555  
 FBN1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for FBN1
Molecular Function
    protein complex binding GO:0032403 IPI
    protein binding GO:0005515 IPI
    extracellular matrix structural constituent GO:0005201 IDA
    integrin binding GO:0005178 IPI
    calcium ion binding GO:0005509 IDA
Biological Process
    sequestering of TGFbeta in extracellular matrix GO:0035583 ISS
    heart development GO:0007507 IMP
    anatomical structure morphogenesis GO:0009653 IBA
    metanephros development GO:0001656 IEA
    extracellular matrix disassembly GO:0022617 TAS
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    skeletal system development GO:0001501 IMP
    sequestering of BMP in extracellular matrix GO:0035582 ISS
    regulation of cellular response to growth factor stimulus GO:0090287 IBA
    extracellular matrix organization GO:0030198 TAS
Subcellular Localization
    basement membrane GO:0005604 IDA
    extracellular matrix GO:0031012 IDA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    extracellular region GO:0005576 TAS
    proteinaceous extracellular matrix GO:0005578 IDA
    microfibril GO:0001527 IDA
 Experiment description of studies that identified FBN1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for FBN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MFAP5  
Invivo Homo sapiens
2 VCAN 1462
Reconstituted Complex Homo sapiens
3 DCN 1634
Invivo Homo sapiens
4 ELN  
Reconstituted Complex Homo sapiens
5 CALR 811
Invivo Homo sapiens
6 FBLN2 2199
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
7 FBN2 2201
Invivo Homo sapiens
8 MFAP2  
Reconstituted Complex Homo sapiens
9 MYOC 4653
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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