 Experiment description of studies that identified Gnb2 in sEVs |
| 1 |
| Experiment ID |
89 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20424270
|
| Organism |
Rattus norvegicus |
| Experiment description |
Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. |
| Authors |
"Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M." |
| Journal name |
JLR
|
| Publication year |
2010 |
| Sample |
Basophilic leukemia cells |
| Sample name |
RBL-2H3 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS |
|
|
| 2 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
73 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
18494037
|
| Organism |
Mus musculus |
| Experiment description |
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. |
| Authors |
"Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ" |
| Journal name |
ELEC
|
| Publication year |
2008 |
| Sample |
Embryonic fibroblasts |
| Sample name |
NIH3T3 |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA]
Western blotting |
|
|
| 4 |
| Experiment ID |
214 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
Leishmania-infected-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
215 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
LPS-treated-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 6 |
| Experiment ID |
216 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
Normal-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 7 |
| Experiment ID |
33 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21136642
|
| Organism |
Mus musculus |
| Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
| Authors |
"Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J" |
| Journal name |
PROTEOMICS_CL
|
| Publication year |
2007 |
| Sample |
Oligodendrocytes |
| Sample name |
Oligodendrocytes
Oli-neu |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy |
|
|
| 8 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
