Gene description for MYO3B
Gene name myosin IIIB
Gene symbol MYO3B
Other names/aliases -
Species Homo sapiens
 Database cross references - MYO3B
ExoCarta ExoCarta_140469
Vesiclepedia VP_140469
Entrez Gene 140469
HGNC 15576
MIM 610040
UniProt Q8WXR4  
 MYO3B identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MYO3B
Molecular Function
    microfilament motor activity GO:0000146 IBA
    actin binding GO:0003779 IEA
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    visual perception GO:0007601 IEA
    sensory perception of sound GO:0007605 IBA
    sensory perception of sound GO:0007605 ISS
    regulation of actin filament length GO:0030832 IBA
    positive regulation of filopodium assembly GO:0051491 IBA
    cochlea morphogenesis GO:0090103 ISS
Subcellular Localization
    photoreceptor inner segment GO:0001917 IBA
    cytoplasm GO:0005737 IEA
    myosin complex GO:0016459 IEA
    stereocilium tip GO:0032426 IBA
    stereocilium tip GO:0032426 ISS
    filopodium tip GO:0032433 IBA
 Experiment description of studies that identified MYO3B in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MYO3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNNC1  
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
4 TFCP2 7024
Affinity Capture-MS Homo sapiens
5 DHFR 1719
Affinity Capture-MS Homo sapiens
6 KIF20A 10112
Affinity Capture-MS Homo sapiens
7 MYC  
Dosage Lethality Homo sapiens
8 YAP1 10413
Affinity Capture-MS Homo sapiens
9 CDC73  
Affinity Capture-MS Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
12 MYL12B 103910
Affinity Capture-MS Homo sapiens
13 OPALIN  
Affinity Capture-MS Homo sapiens
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