Gene description for Aco2
Gene name aconitase 2, mitochondrial
Gene symbol Aco2
Other names/aliases Aco-2
Aco3
D10Wsu183e
Species Mus musculus
 Database cross references - Aco2
ExoCarta ExoCarta_11429
Vesiclepedia VP_11429
Entrez Gene 11429
UniProt Q99KI0  
 Aco2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aco2
Molecular Function
    aconitate hydratase activity GO:0003994 IBA
    aconitate hydratase activity GO:0003994 IDA
    aconitate hydratase activity GO:0003994 ISO
    iron ion binding GO:0005506 ISO
    3 iron, 4 sulfur cluster binding GO:0051538 IEA
    3 iron, 4 sulfur cluster binding GO:0051538 ISO
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 ISO
Biological Process
    liver development GO:0001889 IEA
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 IGI
    tricarboxylic acid cycle GO:0006099 ISO
    tricarboxylic acid cycle GO:0006099 ISO
    citrate metabolic process GO:0006101 ISO
    citrate metabolic process GO:0006101 ISO
    isocitrate metabolic process GO:0006102 IEA
    isocitrate metabolic process GO:0006102 ISO
    response to isolation stress GO:0035900 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IBA
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Aco2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aco2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Acn9  
Co-fractionation Mus musculus
2 Got2 14719
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
3 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Apoo  
Co-fractionation Mus musculus
7 Atp5d  
Co-fractionation Mus musculus
8 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Slc25a4 11739
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
11 Ttn  
Cross-Linking-MS (XL-MS) Mus musculus
12 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Tfe3  
Affinity Capture-MS Mus musculus
14 Ndufab1  
Co-fractionation Mus musculus
15 Ndufa12  
Co-fractionation Mus musculus
16 Atxn1  
Proximity Label-MS Mus musculus
17 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
18 Kctd13  
Affinity Capture-MS Mus musculus
19 Thrb  
Affinity Capture-MS Mus musculus
20 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Ech1  
Cross-Linking-MS (XL-MS) Mus musculus
22 Ndufs6  
Co-fractionation Mus musculus
23 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 Tia1  
Affinity Capture-MS Mus musculus
25 Atp5l  
Co-fractionation Mus musculus
26 Sucla2  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
27 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
28 Kcnma1  
Affinity Capture-MS Mus musculus
29 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
31 Mettl21e  
Affinity Capture-MS Mus musculus
32 Atp5a1 11946
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
33 MAPT  
Reconstituted Complex Homo sapiens
34 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
35 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
36 Fh1 14194
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
37 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Cox5b 12859
Cross-Linking-MS (XL-MS) Mus musculus
39 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
40 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
41 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
42 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
43 Idh3b 170718
Cross-Linking-MS (XL-MS) Mus musculus
44 Sdhb 67680
Co-fractionation Mus musculus
45 Ndufv1  
Cross-Linking-MS (XL-MS) Mus musculus
46 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
47 Uqcrc2 67003
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
48 Atg16l1  
Affinity Capture-MS Mus musculus
49 Dlst  
Co-fractionation Mus musculus
50 Mdh2 17448
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
51 Uqcrfs1 66694
Co-fractionation Mus musculus
52 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
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