Gene description for NEK6
Gene name NIMA-related kinase 6
Gene symbol NEK6
Other names/aliases SID6-1512
Species Homo sapiens
 Database cross references - NEK6
ExoCarta ExoCarta_10783
Vesiclepedia VP_10783
Entrez Gene 10783
HGNC 7749
MIM 604884
UniProt Q9HC98  
 NEK6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NEK6
Molecular Function
    magnesium ion binding GO:0000287 IDA
    transcription corepressor binding GO:0001222 IPI
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    kinesin binding GO:0019894 IPI
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    apoptotic process GO:0006915 IEA
    mitotic spindle organization GO:0007052 TAS
    chromosome segregation GO:0007059 IBA
    chromosome segregation GO:0007059 IDA
    mitotic nuclear membrane disassembly GO:0007077 TAS
    regulation of mitotic cell cycle GO:0007346 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IBA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    protein autophosphorylation GO:0046777 IDA
    spindle assembly GO:0051225 TAS
    cell division GO:0051301 IEA
    regulation of cellular senescence GO:2000772 TAS
Subcellular Localization
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    nuclear speck GO:0016607 IEA
    protein-containing complex GO:0032991 IDA
    centriolar satellite GO:0034451 IDA
 Experiment description of studies that identified NEK6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NEK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HACD3 51495
Affinity Capture-MS Homo sapiens
2 MCM7 4176
Affinity Capture-MS Homo sapiens
3 SMC3 9126
Affinity Capture-MS Homo sapiens
4 FHL5 9457
Two-hybrid Homo sapiens
5 ZNF24  
Two-hybrid Homo sapiens
6 NECAB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 XIAP  
Two-hybrid Homo sapiens
8 ZNF438  
Two-hybrid Homo sapiens
9 CCDC36  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FAM208B 54906
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 CTBP2 1488
Two-hybrid Homo sapiens
13 AES 166
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 SPERT  
Two-hybrid Homo sapiens
15 NFATC1 4772
Affinity Capture-MS Homo sapiens
16 GFAP 2670
Two-hybrid Homo sapiens
17 BSCL2  
Affinity Capture-MS Homo sapiens
18 USP9X 8239
Affinity Capture-MS Homo sapiens
19 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GCN1L1 10985
Affinity Capture-MS Homo sapiens
21 TRIM54  
Two-hybrid Homo sapiens
22 KDELR1 10945
Affinity Capture-MS Homo sapiens
23 LRPPRC 10128
Affinity Capture-MS Homo sapiens
24 NEK7 140609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SLC18A2  
Affinity Capture-MS Homo sapiens
26 PBX4  
Two-hybrid Homo sapiens
27 GOLGA6L2  
Two-hybrid Homo sapiens
28 PRDX3 10935
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
29 TCF12  
Two-hybrid Homo sapiens
30 PCID2 55795
Affinity Capture-MS Homo sapiens
31 SPRTN  
Affinity Capture-MS Homo sapiens
32 KRT40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 FAM220A  
Two-hybrid Homo sapiens
34 CDH16  
Affinity Capture-MS Homo sapiens
35 NUP93 9688
Affinity Capture-MS Homo sapiens
36 KRT31 3881
Two-hybrid Homo sapiens
37 DYNLL1 8655
Affinity Capture-MS Homo sapiens
38 PHF1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
39 EML3 256364
Affinity Capture-MS Homo sapiens
40 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ANKRA2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
42 NUDT16L1 84309
Two-hybrid Homo sapiens
43 NIF3L1 60491
Two-hybrid Homo sapiens
44 FAM154A  
Two-hybrid Homo sapiens
45 EML2 24139
Affinity Capture-MS Homo sapiens
46 KRT8 3856
Two-hybrid Homo sapiens
47 KRT34  
Two-hybrid Homo sapiens
48 ARHGAP33 115703
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
49 SLC1A5 6510
Affinity Capture-MS Homo sapiens
50 EML4 27436
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
51 METTL15  
Two-hybrid Homo sapiens
52 MYOZ3  
Two-hybrid Homo sapiens
53 RIMBP3C  
Two-hybrid Homo sapiens
54 CCAR2 57805
Affinity Capture-MS Homo sapiens
55 DERA 51071
Co-fractionation Homo sapiens
56 PPP1R16A 84988
Two-hybrid Homo sapiens
57 KRTAP13-1  
Two-hybrid Homo sapiens
58 ATF4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
59 CFP  
Two-hybrid Homo sapiens
60 PRDM16 63976
Two-hybrid Homo sapiens
61 PSMD2 5708
Affinity Capture-MS Homo sapiens
62 KRTAP10-8  
Two-hybrid Homo sapiens
63 GPR182  
Affinity Capture-MS Homo sapiens
64 BEND7 222389
Two-hybrid Homo sapiens
65 MEOX2  
Two-hybrid Homo sapiens
66 CEP76  
Two-hybrid Homo sapiens
67 FOXJ3  
Two-hybrid Homo sapiens
68 CEBPA  
Protein-peptide Homo sapiens
69 ZNF688  
Two-hybrid Homo sapiens
70 DPH2 1802
Two-hybrid Homo sapiens
71 ARRB1 408
Two-hybrid Homo sapiens
72 PTN  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
73 RBBP6 5930
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
74 HSD17B14  
Two-hybrid Homo sapiens
75 KATNBL1  
Two-hybrid Homo sapiens
76 IKZF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 CCDC120  
Two-hybrid Homo sapiens
78 MYOG  
Two-hybrid Homo sapiens
79 KRT75 9119
Two-hybrid Homo sapiens
80 DES 1674
Two-hybrid Homo sapiens
81 BHLHE40  
Two-hybrid Homo sapiens
82 SH3RF2  
Two-hybrid Homo sapiens
83 HOXA1 3198
Two-hybrid Homo sapiens
84 IKZF1  
Two-hybrid Homo sapiens
85 ERCC6L2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
86 MYO1C 4641
Affinity Capture-MS Homo sapiens
87 ZCCHC7  
Two-hybrid Homo sapiens
88 CDC42 998
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
89 LZTS2 84445
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 COG6 57511
Two-hybrid Homo sapiens
91 NEFL 4747
Two-hybrid Homo sapiens
92 GIGYF1  
Two-hybrid Homo sapiens
93 SLC22A2 6582
Affinity Capture-MS Homo sapiens
94 CCDC85B  
Two-hybrid Homo sapiens
95 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 TRAF2 7186
Two-hybrid Homo sapiens
97 SORBS3 10174
Two-hybrid Homo sapiens
98 ZNF587  
Two-hybrid Homo sapiens
99 ZC2HC1C  
Two-hybrid Homo sapiens
100 ENTHD2  
Two-hybrid Homo sapiens
101 GRIP1  
Two-hybrid Homo sapiens
102 C19orf45  
Two-hybrid Homo sapiens
103 SMUG1  
Two-hybrid Homo sapiens
104 GYPA  
Affinity Capture-MS Homo sapiens
105 CIR1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
106 TNK2 10188
Two-hybrid Homo sapiens
107 KRTAP13-3  
Two-hybrid Homo sapiens
108 TMEM9 252839
Affinity Capture-MS Homo sapiens
109 EFHC2  
Two-hybrid Homo sapiens
110 PTPRD 5789
Reconstituted Complex Homo sapiens
111 HOMEZ  
Two-hybrid Homo sapiens
112 NEK6 10783
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
113 RUVBL2 10856
Affinity Capture-MS Homo sapiens
114 HOXB6 3216
Two-hybrid Homo sapiens
115 INCA1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
116 CDC37 11140
Affinity Capture-MS Homo sapiens
117 GLIS3  
Two-hybrid Homo sapiens
118 FANCI 55215
Affinity Capture-MS Homo sapiens
119 RBAK  
Two-hybrid Homo sapiens
120 UBXN11  
Two-hybrid Homo sapiens
121 DDIT4L  
Two-hybrid Homo sapiens
122 KIAA1328  
Two-hybrid Homo sapiens
123 PRAM1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
124 PRICKLE4  
Two-hybrid Homo sapiens
125 SPRY3  
Two-hybrid Homo sapiens
126 ZFP90  
Two-hybrid Homo sapiens
127 XPO5 57510
Affinity Capture-MS Homo sapiens
128 TCF4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 TRIM23 373
Two-hybrid Homo sapiens
130 IPO4 79711
Affinity Capture-MS Homo sapiens
131 TRIP4 9325
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
132 RELB  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
133 RPS7 6201
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
134 PLEKHA7 144100
Two-hybrid Homo sapiens
135 CCDC136  
Two-hybrid Homo sapiens
136 MBD3L1  
Two-hybrid Homo sapiens
137 NEK9 91754
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 ZNF774  
Two-hybrid Homo sapiens
139 KCTD5 54442
Two-hybrid Homo sapiens
140 LNX1  
Two-hybrid Homo sapiens
141 PAK6 56924
Two-hybrid Homo sapiens
View the network image/svg+xml



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