Gene description for NCALD
Gene name neurocalcin delta
Gene symbol NCALD
Other names/aliases -
Species Homo sapiens
 Database cross references - NCALD
ExoCarta ExoCarta_83988
Entrez Gene 83988
HGNC 7655
MIM 606722
UniProt P61601  
 NCALD identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NCALD
Molecular Function
    tubulin binding GO:0015631 IDA
    protein binding GO:0005515 IPI
    alpha-tubulin binding GO:0043014 IEA
    clathrin binding GO:0030276 IDA
    calcium ion binding GO:0005509 IEA
    actin binding GO:0003779 IDA
Biological Process
    regulation of systemic arterial blood pressure GO:0003073 IEA
    vesicle-mediated transport GO:0016192 NAS
    calcium-mediated signaling GO:0019722 IEA
    synaptic transmission GO:0007268 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    clathrin coat of trans-Golgi network vesicle GO:0030130 NAS
    intracellular GO:0005622 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified NCALD in exosomes
1
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCALD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA1A 7846
Affinity Capture-MS Homo sapiens
2 TUBB2A 7280
Affinity Capture-MS Homo sapiens
3 DTX2  
Two-hybrid Homo sapiens
4 ACTB 60
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which NCALD is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor TAS Reactome





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