Gene description for WNT11
Gene name wingless-type MMTV integration site family, member 11
Gene symbol WNT11
Other names/aliases HWNT11
Species Homo sapiens
 Database cross references - WNT11
ExoCarta ExoCarta_7481
Vesiclepedia VP_7481
Entrez Gene 7481
HGNC 12776
MIM 603699
UniProt O96014  
 WNT11 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for WNT11
Molecular Function
    frizzled binding GO:0005109 IBA
    frizzled binding GO:0005109 NAS
    cytokine activity GO:0005125 IBA
    protein binding GO:0005515 IPI
    protein kinase activator activity GO:0030295 IMP
Biological Process
    osteoblast differentiation GO:0001649 IEA
    epithelial to mesenchymal transition GO:0001837 IEA
    primary heart field specification GO:0003138 IEA
    secondary heart field specification GO:0003139 IEA
    outflow tract morphogenesis GO:0003151 IEA
    atrial septum development GO:0003283 IEA
    signal transduction GO:0007165 IMP
    Wnt signaling pathway, calcium modulating pathway GO:0007223 NAS
    mesenchymal cell proliferation GO:0010463 IEA
    neuron differentiation GO:0030182 IBA
    neuron differentiation GO:0030182 ISS
    bone mineralization GO:0030282 IEA
    adrenal gland development GO:0030325 IEP
    response to nutrient levels GO:0031667 IEA
    positive regulation of transforming growth factor beta2 production GO:0032915 IEA
    intracellular signal transduction GO:0035556 IMP
    cell fate commitment GO:0045165 IBA
    maintenance of epithelial cell apical/basal polarity GO:0045199 IEA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    paraxial mesoderm formation GO:0048341 IEA
    notochord morphogenesis GO:0048570 IEA
    embryonic skeletal system development GO:0048706 IEP
    artery morphogenesis GO:0048844 IEA
    convergent extension involved in axis elongation GO:0060028 IEA
    canonical Wnt signaling pathway GO:0060070 IBA
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 NAS
    cloacal septation GO:0060197 IEP
    ventricular septum morphogenesis GO:0060412 IEA
    lung-associated mesenchyme development GO:0060484 IEP
    ureteric bud morphogenesis GO:0060675 IEP
    negative regulation of cartilage development GO:0061037 NAS
    somite development GO:0061053 IEA
    neuroendocrine cell differentiation GO:0061101 IMP
    secondary palate development GO:0062009 IMP
    bicellular tight junction assembly GO:0070830 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to retinoic acid GO:0071300 ISS
    stem cell proliferation GO:0072089 IEA
    mesonephric duct development GO:0072177 IEP
    negative regulation of mesenchymal cell proliferation GO:0072201 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    negative regulation of fibroblast growth factor production GO:0090272 IEA
    epithelial cell apoptotic process GO:1904019 IEA
    positive regulation of epithelial cell apoptotic process GO:1904037 IEA
    negative regulation of stem cell proliferation GO:2000647 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IDA
    extracellular matrix GO:0031012 IEA
 Experiment description of studies that identified WNT11 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for WNT11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 TMED7 51014
Affinity Capture-MS Homo sapiens
3 CANX 821
Affinity Capture-MS Homo sapiens
4 PDGFRA 5156
Affinity Capture-MS Homo sapiens
5 MDFI  
Two-hybrid Homo sapiens
6 LDLRAD1  
Affinity Capture-MS Homo sapiens
7 MKRN2 23609
Affinity Capture-RNA Homo sapiens
8 WNT16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 C1orf54  
Affinity Capture-MS Homo sapiens
11 DDX58 23586
Affinity Capture-RNA Homo sapiens
12 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KRTAP10-9  
Two-hybrid Homo sapiens
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