Gene description for TFPI
Gene name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Gene symbol TFPI
Other names/aliases EPI
LACI
TFI
TFPI1
Species Homo sapiens
 Database cross references - TFPI
ExoCarta ExoCarta_7035
Entrez Gene 7035
HGNC 11760
MIM 152310
UniProt P10646  
 TFPI identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
 Gene ontology annotations for TFPI
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    endopeptidase inhibitor activity GO:0004866 TAS
Biological Process
    negative regulation of endopeptidase activity GO:0010951 TAS
    blood coagulation, extrinsic pathway GO:0007598 TAS
    blood coagulation GO:0007596 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum GO:0005783 IEA
    anchored component of membrane GO:0031225 IEA
    organelle membrane GO:0031090 IEA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
 Experiment description of studies that identified TFPI in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 144
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90|RAB5
EV Cytosolic markers
CD9
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
3
Experiment ID 145
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90|RAB5
EV Cytosolic markers
CD10
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
 Protein-protein interactions for TFPI
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THBS1 7057
Invitro Homo sapiens
2 F10 2159
Invitro Homo sapiens
3 PRSS3 5646
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which TFPI is involved
PathwayEvidenceSource
Extrinsic Pathway of Fibrin Clot Formation TAS Reactome





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