Gene description for Eef1d
Gene name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Gene symbol Eef1d
Other names/aliases EF-1-delta
Species Mus musculus
 Database cross references - Eef1d
ExoCarta ExoCarta_66656
Vesiclepedia VP_66656
Entrez Gene 66656
UniProt P57776  
 Eef1d identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eef1d
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA binding GO:0003677 IEA
    translation elongation factor activity GO:0003746 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    heat shock protein binding GO:0031072 ISO
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    translational elongation GO:0006414 IBA
    translational elongation GO:0006414 IEA
    cellular response to heat GO:0034605 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    cellular response to ionizing radiation GO:0071479 ISO
Subcellular Localization
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    eukaryotic translation elongation factor 1 complex GO:0005853 IEA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Eef1d in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eef1d
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Vars 22321
Co-fractionation Mus musculus
3 Atxn1  
Proximity Label-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Eef1d 66656
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Fos  
Reconstituted Complex Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Eef1d is involved
PathwayEvidenceSource
Eukaryotic Translation Elongation IEA Reactome
Metabolism of proteins IEA Reactome
Translation IEA Reactome





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