Gene description for CCL20
Gene name chemokine (C-C motif) ligand 20
Gene symbol CCL20
Other names/aliases CKb4
Exodus
LARC
MIP-3-alpha
MIP-3a
MIP3A
SCYA20
ST38
Species Homo sapiens
 Database cross references - CCL20
ExoCarta ExoCarta_6364
Entrez Gene 6364
HGNC 10619
MIM 601960
UniProt P78556  
 CCL20 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CCL20
Molecular Function
    protein binding GO:0005515 IPI
    chemokine activity GO:0008009 IEA
Biological Process
    positive regulation of interleukin-1 alpha biosynthetic process GO:0050726 IEA
    immune response GO:0006955 IEA
    cell-cell signaling GO:0007267 TAS
    inflammatory response GO:0006954 IEA
    wound healing GO:0042060 IEA
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 IEA
    cellular response to lipoteichoic acid GO:0071223 IEA
    defense response to bacterium GO:0042742 IEA
    cell chemotaxis GO:0060326 TAS
    chemotaxis GO:0006935 TAS
    positive regulation of T cell migration GO:2000406 IDA
    signal transduction GO:0007165 TAS
    cellular response to lipopolysaccharide GO:0071222 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IEA
 Experiment description of studies that identified CCL20 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CCL20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Invitro Homo sapiens
2 CCR6  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which CCL20 is involved
PathwayEvidenceSource
Chemokine receptors bind chemokines TAS Reactome
G alpha (i) signalling events TAS Reactome





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